Shear stimulated Brillouin microscopy for cell mechanobiology

用于细胞力学生物学的剪切刺激布里渊显微镜

• 批准号: DP190101973
• 负责人: A/Prof Irina Kabakova
• 金额: $ 23.35万
• 依托单位: University of Technology Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-01-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP190101973
• 关键词: Shear; stimulated; Brillouin; microscopy; cell

This project aims to develop novel technology for non-contact imaging of micro-mechanical properties in cells and tissues to answer fundamental questions of cell mechnanobiology. Based on principles of Brillouin light scattering, the project takes advantage of a radio-frequency lock-in detection scheme. The project will result in a real-time, high-sensitivity, non-contact 3D imaging solution for spatial characterisation of cell's local stiffness and compressibility. This will underpin the advancement of knowledge in the area of cell mechanobiology and the investigation of diseases, where microscale changes in cell mechanical properties lead to cell dysfunction and apoptosis.

该项目旨在开发细胞和组织中微观力学特性的非接触成像新技术，以回答细胞机械生物学的基本问题。该项目基于布里渊光散射原理，采用射频锁定检测方案。该项目将产生一种实时、高灵敏度、非接触式3D成像解决方案，用于细胞局部刚度和可压缩性的空间表征。这将支持细胞机械生物学和疾病研究领域的知识进步，其中细胞机械特性的微观变化导致细胞功能障碍和细胞凋亡。