GLUTAMINE METABOLISM IN HUMAN NEONATES

这项提案的总体目标是阐明 新生儿的谷氨酰胺代谢，并确定早产儿和 外科手术的压力改变了这些途径。谷氨酰胺，一种重要的燃料 淋巴细胞和肠上皮细胞，可能是不可或缺的氨基酸 酸对早产儿和手术压力过大的婴儿的影响。因此， 测定这些婴儿合成谷氨酰胺的能力和 对体内现有蛋白质释放谷氨酰胺的调节是 很重要。补充谷氨酰胺可能在 支持最佳蛋白质积累，从肠道损伤中恢复，以及 这些特别脆弱的婴儿的免疫功能。具体的 这项提案的目的是确定从头开始谷氨酰胺的比率 谷氨酰胺从体内蛋白质的合成和释放被改变 早产与手术应激：1)基础状态2)反应 提供肠外营养和3)回应 提供补充谷氨酰胺的肠外营养。这些 将使用稳定的同位素示踪剂实现具体目标。表演 这些研究将为了解谷氨酰胺和 最终，可以作为改善营养管理的基础 早产儿和手术压力过大的婴儿。

The overall objective of this proposal is to elucidate the pathways of glutamine metabolism in neonates and to determine how prematurity and surgical stress alter these pathways. Glutamine, an important fuel for lymphocytes and intestinal epithelial cells, may be an indispensable amino acid in premature and surgically stressed infants. Consequently, determining the capacity of these infants to synthesize glutamine and the regulation of the release of glutamine from existing body protein is important. Glutamine supplementation may play a critical role in supporting optimal protein accretion, recovery from intestinal injury, and immune function in these particularly vulnerable infants. The specific aims of this proposal are to determine how rates of de novo glutamine synthesis and release of glutamine from body protein are altered by prematurity and surgical stress in: 1) the basal state 2) in response to the provision of parenteral nutrition and 3) in response to the provision of parenteral nutrition with supplemental glutamine. These specific aims will be pursued using stable isotope tracers. Performing these studies will provide insight into the metabolism of glutamine and ultimately, may serve as the basis for improved nutritional management of premature and surgically stressed infants.