ISOLATION OF BIPOLAR CELL RESPONSES IN THE MACAQUE ERG

猕猴 ERG 中双极细胞反应的分离

• 批准号: 2775372
• 负责人: Jameel Ahmed
• 金额: $ 2.07万
• 依托单位: UNIVERSITY OF HOUSTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-29 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2775372
• 关键词: G protein; Macaca mulatta; Muller's cell; behavioral /social science research tag; cats; dark adaptation; diagnosis design /evaluation; electroretinography; eye disorder diagnosis; eye pharmacology; glutamates; inhibitor /antagonist; mathematical model; model design /development; neural information processing; retina disorder; retinal bipolar neuron; stimulant /agonist; visual photoreceptor

There are two major goals of this research. The first is to characterize the bipolar cell contribution to the primate flash electroretinogram (ERG) under both fully dark-adapted conditions, and in the presence of steady adapting backgrounds. The second goal is to use the isolated bipolar cell component to give us information about the role that bipolar cells play in visual signal processing. In order to obtain this understanding of bipolar cell responses, the technique of pharmacological isolation will be used in macaque monkeys, an animal whose retina is very close to that of man. An understanding of bipolar cell responses is important from both a clinical and a basic science point of view. At a basic level, bipolar cells clearly play an important role in retinal signal processing, and yet, because of the technical difficulties of recording form such cells in mammals, it has not been possible to carry out detailed studies of their response properties. Clinically, there are direct and indirect reasons for studying PH in the ERG. Directly, this information would be valuable in interpreting changes in the ERG that occur in diseases that involve bipolar cells themselves, such as congenital stationary night blindness. Indirectly, a good understanding of the characteristics of the PII component would allow better assessment of effects of retinal pathology on other components of the ERG, originating either distal to the bipolar cells (a-wave from photoreceptors affected, for example, by retinitis pigmentosa), or proximal to the bipolar cells (oscillatory potentials, scotopic threshold response from amacrine or ganglion cells affected, for example, by diabetic retinopathy, glaucoma or optic neuritis).

这项研究有两个主要目标。 第一个是 描述双极细胞对灵长类闪光的贡献 完全暗适应条件下的视网膜电图（ERG），以及 在稳定的适应背景下。 第二个目标是 使用隔离的双极电池组件为我们提供有关 双极细胞在视觉信号处理中发挥的作用。 为了 获得对双极细胞反应的理解，该技术 药物分离将用于猕猴（一种动物） 其视网膜与人类非常接近。对双相情感障碍的理解 细胞反应对于临床和基础科学都很重要 观点看法。 在基本层面上，双极细胞显然发挥着 在视网膜信号处理中发挥着重要作用，然而，由于 在哺乳动物中记录此类细胞的技术困难， 无法对他们的反应进行详细研究 特性。临床上，有直接原因和间接原因。 在 ERG 中研究 PH。 直接来说，这些信息很有价值 解释涉及疾病的 ERG 变化 双相细胞本身，例如先天性静止性夜盲症。 间接地，很好地理解PII的特性 组件可以更好地评估视网膜病理学的影响 在 ERG 的其他组成部分上，起源于双极的远端 细胞（来自受视网膜炎等影响的光感受器的 a 波） 色素性），或接近双极细胞（振荡电位， 来自受影响的无长突细胞或神经节细胞的暗视阈值反应， 例如，糖尿病性视网膜病、青光眼或视神经炎）。