MAP BINDING EFFECT ON MICROTUBULE STIFFNESS

图谱结合对微管硬度的影响

• 批准号: 2749747
• 负责人: STEVEN P GROSS
• 金额: $ 3.05万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2749747
• 关键词: biophysics; chemical binding; microtubule associated protein; microtubules

The goal of this proposal is to explore further the interaction between microtubules (MTs) and microtubule-associated proteins (MAPs), particularly the ability of certain MAPs to alter the mechanical properties of MTs. It is possible that one of the key functions of MAP binding is to produce concomitant changes in MT stiffness, affecting neuronal development (MAP2 and Tau) or mitotic processes (MAP4), and there is preliminary evidence that MAP binding does result in an increase of MT stiffness. Details of how MAP binding changes MT mechanical properties, and how MAP phosphorylation alters such effects, are potentially useful in understanding certain neuronal diseases like Alzheimer's disease (where infected patients exhibit a hyperphosphorylated form of Tau), and may also help understand certain details of mitosis, during which MAP4's state of phosphorylation is changed by MPF. To accurately measure MT stiffness, a state-of-the-art dual-beam optical trap will be built for these experiments. The trap will be used to bend individual MTs, by exerting force on beads bound to each end of the MT. Included in the trap design is the ability to accurately measure applied force. Knowing the applied force and resultant curve in the MT will enable the determination of the MT stiffness. These measurements will be carried out in the absence and presence of MAPs, so that their effect on stiffness can be measured. Phosphorylated and genetically engineered MAPs will also be used in these studies, to examine their relative effects.

本提案的目标是进一步探索两者之间的相互作用

项目成果

Dynein-mediated cargo transport in vivo. A switch controls travel distance.

Dynein介导的体内货物运输。开关控制旅行距离。

• DOI: 10.1083/jcb.148.5.945
• 发表时间: 2000-03-06
• 期刊: JOURNAL OF CELL BIOLOGY
• 影响因子: 7.8
• 作者: Gross, S P;Welte, M A;Block, S M;Wieschaus, E F
• 通讯作者: Wieschaus, E F

Coordination of opposite-polarity microtubule motors.

• DOI: 10.1083/jcb.200109047
• 发表时间: 2002-02-18
• 期刊: The Journal of cell biology
• 作者: Gross SP;Welte MA;Block SM;Wieschaus EF
• 通讯作者: Wieschaus EF