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CHLOROPLAST TO NUCLEUS SIGNALING IN ARABIDOPSIS

拟南芥中叶绿体到细胞核的信号转导

基本信息

批准号：
2749744
负责人：
MARCI A SURPIN
金额：
$ 3.02万
依托单位：
SALK INSTITUTE FOR BIOLOGICAL STUDIES
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-08-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/2749744
关键词：
Arabidopsis antibody artificial chromosomes biological signal transduction cell nucleus chloroplasts gene expression gene mutation genetic regulatory element immunoprecipitation molecular cloning nucleic acid sequence plant growth /development plant proteins

项目摘要

Cancer is the result of aberrant cell growth. In order to formulate therapies for the various forms of the disease, it is necessary to understand the molecular mechanisms that regulate cellular development. Arabidopsis thaliana its known to express homologs of oncoproteins; it is also a system amenable to genetic, molecular and biochemical analyses. Therefore, the study of signal transduction pathways in Arabidopsis will yield valuable information about normal cellular development. Organellar biogenesis requires the coordinate expression of nuclear and organelle genes. There is evidence in plants that a chloroplast signal controls the expression of a subset of nuclear genes that functions in photosynthesis. The nature of the signal, and the means by which it is relayed to the nucleus, are unknown chloroplast genomes. gun mutants inappropriately express photosynthesis-related nuclear when chloroplast development is inhibited. I am currently cloning the GUNI gene, and when it is cloned, will characterize the gene product and determine how it helps coordinated nuclear and organellar gene expression. I am also carrying out genetic studies of the gun mutants, and the determining the plastid- responsive cis-acting sequencing in nuclear genes. The results from these experiments will further our understanding of intracellular signal transduction pathways.

癌症是细胞异常生长的结果。为了制定治疗各种形式的疾病，有必要了解调节细胞发育的分子机制。已知拟南芥表达癌蛋白的同源物; 也是一种适合于遗传、分子和生物化学分析的系统。因此，对拟南芥中信号转导途径的研究，产生关于正常细胞发育的有价值的信息。细胞器的生物发生需要细胞核和细胞器基因在植物中有证据表明叶绿体信号控制一部分核基因的表达，光合作用信号的性质，以及它的手段，传递到细胞核的是未知的叶绿体基因组。枪突变体当叶绿体不适当地表达光合作用相关核时发展受到抑制。我目前正在克隆GUNI基因，它被克隆，将表征基因产物，并确定它如何帮助协调细胞核和细胞器基因表达。我也在执行枪突变体的遗传学研究，以及确定质体- 核基因中的顺式作用序列。这些研究的结果实验将进一步加深我们对细胞内信号的理解转导途径。