CONTROL OF SEGMENT-SPECIFIC PREGANGLIONIC AXON OUTGROWTH

节段特异性节前轴突生长的控制

• 批准号: 2750853
• 负责人: CYNTHIA J FOREHAND
• 金额: $ 27.5万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-18 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2750853
• 关键词: axon; chick embryo; electron microscopy; extracellular matrix; fibroblast growth factor; immunocytochemistry; innervation; laboratory mouse; neurogenesis; preganglionic fiber; receptor; receptor expression; spinal cord; sympathetic ganglion

DESCRIPTION (Applicant's Abstract): Sympathetic preganglionic neurons exhibit both an intrasegmental pattern, in which cells destined to project caudally into the sympathetic chain are located rostrally in the segment, while those that project caudally into the sympathetic chain are located caudally in the segment, and an intersegmental pattern, in which the number of rostrally or caudally projecting cells within a spinal segment is determined by the segmental position along the rostrocaudal axis. There is evidence to indicate that preganglionic cells are of two district groups, based on their pattern of projection into the sympathetic chain. First, individual cells project either rostrally or caudally into the sympathetic chain, but rarely in both directions. Second, manipulations of the periphery, such as application of exogenous retinoic acid (RA) or transposition or somites along the rostrocaudal axis, differentially alter the preganglionic cell pools. These studies have provided the first demonstration that cell proliferation and/or survival in the preganglionic cell column in the thoracic spinal cord is influenced by peripheral cues early in development. We hypothesize that preganglionic neurons have an inherent identity as either rostrally or caudally projecting cells. The specific aims of this application are designed to address when the preganglionic pools become different, what and where the molecular signals are that regulate the size of preganglionic populations, the way in which the preganglionic neurons respond to peripheral cues to make appropriate pathway choices, and what the implications of disrupting the normal regulation of pattern in this system are for the functional organization of the autonomic nervous system. The specific aims are: 1. To determine whether preganglionic neurons are fated to project either rostrally or caudally by lineage restrictions in early development. 2. To determine the way in which exogenous FGF treatment regulates the size of preganglionic populations. 3. To determine whether differential expression of receptors for extracellular matrix molecules distinguishes classes of preganglionic neuron and 4. To determine whether alterations in the number of either class of sympathetic preganglionic neurons is associated with concomitant alterations in either cell number or innervation density within the paravertebral sympathetic chain ganglia. Understanding the regulation of the organization of this system has the potential to provide a unique situation for studying the general way in which topographic maps of the body are matched to various levels of the neuraxis.

描述（申请人摘要）：交感神经节前神经元 表现出段内模式，其中细胞注定要投射 尾部进入交感神经链位于节段的吻侧， 而尾部投射到交感神经链的那些位于 尾部在节段中，以及节间模式，其中数量 脊柱节段内的头侧或尾侧突出的细胞是 由沿 rostrocaudal 轴的节段位置确定。 有 有证据表明节前细胞属于两个区组， 基于它们投射到交感神经链的模式。 第一的， 单个细胞从头侧或尾侧投射到交感神经中 链，但很少是双向的。 二、操纵 外周，如应用外源性维A酸（RA）或 沿轴尾轴的转位或体节，有差异地改变 节前细胞池。 这些研究首次提供了 证明节前细胞增殖和/或存活 胸脊髓的细胞柱受外周信号的影响 处于开发早期。 我们假设节前神经元具有 作为嘴部或尾部突出细胞的固有特性。 这 该应用程序的具体目标旨在解决 节前池变得不同，分子信号是什么以及在哪里 是调节节前群体大小的方式 节前神经元对周围信号作出反应，做出适当的反应 途径选择，以及扰乱正常的影响 该系统模式的规定是为了功能组织 自主神经系统。 具体目标是： 1. 确定 节前神经元是否注定要投射到吻侧或 尾部受到早期发育中的谱系限制。 2. 确定 外源性 FGF 治疗调节节前细胞大小的方式 人口。 3. 判断受体是否差异表达 对于细胞外基质分子，区分神经节前的类别 神经元和 4. 确定任一数量是否发生变化 交感神经节前神经元的类别与伴随的 细胞数量或神经支配密度的改变 椎旁交感神经节。 了解监管 该系统的组织有可能提供独特的 研究人体地形图的一般方法的情况 与不同层次的神经轴相匹配。