OVEREXPRESSION OF NERVE GROWTH FACTOR

神经生长因子过度表达

• 批准号: 2669021
• 负责人: BRIAN M DAVIS
• 金额: $ 25.19万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-05-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2669021
• 关键词: afferent nerve; animal genetic material tag; developmental genetics; developmental neurobiology; gene expression; genetically modified animals; laboratory mouse; nerve injury; neurogenesis; neurogenetics; neurotrophic factors; neutralizing antibody; peripheral nervous system; ventral column; wound healing

DESCRIPTION: Neurotrophins are known to control not only neuronal survival, but may modulate phenotypic differentiation and adult neuropathic pain. In transgenic mice overexpressing NT-3 or NGF, changes are noted in sensory neuron number, projections and pain threshold. The collaborating lab has the ability to determine the "Comprehensive phenotype" of adult sensory neurons, using intracellular recording and labeling. These techniques will be used to examine individual sensory neurons of control and transgenic mice to assess if neurotrophin overexpression alters the phenotype (aims 1 and 2). It is also hypothesized that neuropathic pain recapitulates neurotrophin dependent processes. In aim 3, the phenotype of neurons responsible for neuropathic pain will be characterized, and antineurotrophin antisera will be used to block formation of the sympathetic/sensory neuron connections thought to mediate neuropathic pain.

描述：众所周知，神经营养素不仅可以控制神经元的存活， 但可能调节表型分化和成人神经性疼痛。 在 过度表达 NT-3 或 NGF 的转基因小鼠，感觉发生变化 神经元数量、投射和痛阈。 合作实验室有 确定成人感官“综合表型”的能力 神经元，使用细胞内记录和标记。 这些技术将 用于检查对照小鼠和转基因小鼠的单个感觉神经元 评估神经营养素过度表达是否会改变表型（目标 1 和 2）。 还假设神经性疼痛概括了 神经营养素依赖性过程。 在目标 3 中，神经元的表型 负责神经性疼痛的特征将被表征，并且抗神经营养因子 抗血清将用于阻止交感神经/感觉神经元的形成 被认为可以介导神经性疼痛的连接。