FUNCTIONAL ASPECTS OF AN ENERGY TRANSDUCING COMPLEX

能量转换复合物的功能方面

• 批准号: 6018453
• 负责人: RICHARD MALKIN
• 金额: $ 18.74万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-07-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6018453
• 关键词: Chlamydomonas; chemical kinetics; cytochromes; electron transport; intermolecular interaction; membrane transport proteins; plant genetics; protein kinase; protein transport; site directed mutagenesis; water channel

DESCRIPTION: Cytochrome bc/bf complexes play a critical role in energy metabolism of cells by coupling redox reactions to the translocation of protons across a biological membrane for the generation of a proton motive force that is used to synthesize ATP. The main working model for the operation of these complexes is the Q-cycle which involves oxidation and reduction of quinone/quinol at two independent sites on the complex. This proposal has focused on structural and functional aspects of the cytochrome b6f complex in oxygenic photosynthetic membranes. They will define the role of individual components in the reactions of the complex as well as in proposed roles related to the assembly/stabilization of the complex. They will consider structural features of the holocomplex as well as study an important regulatory activity that has recently been identified. In terms of the complex in situ, they will measure electron transfer reactions using a flash-kinetic approach, in order to evaluate proposed mechanisms. They will consider in some detail the molecular nature of electron transfer proteins and other non-prosthetic binding proteins in the complex, using the green alga, Chlamydomonas, in a molecular genetic approach. This will involve site-directed mutagenesis for modification of specific amino acids in these proteins to define their functions, taking advantage, in one case, of the structural information that has recently been provided for cytochrome f. Finally, they will attempt to define the role of the cytochrome b6f complex in regulating a kinase that phosphorylates membrane proteins. These studies will provide important findings relating to the function of energy transducing membrane protein complexes.

描述：细胞色素bc/bf复合物在能量中起关键作用。 通过将氧化还原反应偶联到 质子穿过生物膜以产生质子动力 用于合成ATP的力。 的主要工作模型 这些络合物的操作是Q循环，其涉及氧化和 醌/醌醇在复合物上两个独立位点的还原。 这 该提案集中在细胞色素的结构和功能方面， 含氧光合膜中的B6 F复合物。 他们将定义角色 在复合物的反应中， 与建筑群的集结/稳定有关的拟议作用。 他们 将考虑全复合体的结构特征，并研究 最近发现的重要监管活动。 方面 他们将测量电子转移反应， 闪光动力学方法，以评估拟议的机制。 他们 我将详细讨论电子转移的分子性质 蛋白质和其他非假体结合蛋白的复合物，使用 绿色衣原体，分子遗传学方法。 这将 涉及定点诱变以修饰特定氨基酸 在这些蛋白质中定义它们的功能，在一种情况下， 最近提供的细胞色素 F.最后，他们将试图确定细胞色素b6 f的作用， 在调节磷酸化膜蛋白的激酶中的复合物。 这些 研究将提供有关能量功能的重要发现， 转导膜蛋白复合物。