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REDUCED FOLATE TRANSPORT AND ANTIFOLATE RESISTANCE

REDUCED FOLATE TRANSPORT AND ANTIFOLATE RESISTANCE
减少叶酸转运和抗叶酸抗药性
批准号：
2733001
负责人：
Peter William Melera
金额：
$23.59万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
1990
资助国家：
美国
项目状态：
已结题
起止时间：
1990-05-01 至 1999-12-15
来源：
https://reporter.nih.gov/project-details/2733001
关键词：
SDS polyacrylamide gel electrophoresis binding proteins complementary DNA dihydrofolate reductase drug resistance folate antagonist gene expression genetic library glycoproteins hamsters membrane transport proteins methotrexate mutant polymerase chain reaction restriction mapping tissue /cell culture transfection /expression vector vitamin antagonist vitamin metabolism

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中文摘要
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英文摘要
DESCRIPTION: This project is focused upon determining the molecular genetic basis for alterations in transport that render cells resistant to antifolates by preventing drug accumulation. A full length cDNA will be cloned by preparing an expression library from the Chinese hamster lung cell line DC- 3F/FA3 that appears to overexpress a gene for a 43.2 kd glycoprotein associated with enhanced folate uptake. Functional interactions of this and the hp58 gene product will be studied and used to isolate putative mutant transporters from existing cell lines.
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DHFR MEDIATED ANTIFOLATE RESISTANCE IN MAMMALIAN CELLS
  • 批准号：
    3193707
  • 项目类别：
  • 资助金额：
    $18.52万
  • 财政年份：
    1990
  • 负责人：
    Peter William Melera
  • 依托单位：
    UNIVERSITY OF MARYLAND BALTIMORE
REDUCED FOLATE TRANSPORT AND ANTIFOLATE RESISTANCE
  • 批准号：
    2442974
  • 项目类别：
  • 资助金额：
    $22.68万
  • 财政年份：
    1990
  • 负责人：
    Peter William Melera
  • 依托单位：
    UNIVERSITY OF MARYLAND BALTIMORE
REDUCED FOLATE TRANSPORT AND ANTIFOLATE RESISTANCE
  • 批准号：
    2093333
  • 项目类别：
  • 资助金额：
    $21.81万
  • 财政年份：
    1990
  • 负责人：
    Peter William Melera
  • 依托单位：
    UNIVERSITY OF MARYLAND BALTIMORE
DHFR MEDIATED ANTIFOLATE RESISTANCE IN MAMMALIAN CELLS
  • 批准号：
    3193706
  • 项目类别：
  • 资助金额：
    $17.19万
  • 财政年份：
    1990
  • 负责人：
    Peter William Melera
  • 依托单位：
    UNIVERSITY OF MARYLAND BALTIMORE
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