REDUCED FOLATE TRANSPORT AND ANTIFOLATE RESISTANCE

减少叶酸转运和抗叶酸抗药性

• 批准号: 2733001
• 负责人: Peter William Melera
• 金额: $ 23.59万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-05-01 至 1999-12-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2733001
• 关键词: SDS polyacrylamide gel electrophoresis; binding proteins; complementary DNA; dihydrofolate reductase; drug resistance; folate antagonist; gene expression; genetic library; glycoproteins; hamsters; membrane transport proteins; methotrexate; mutant; polymerase chain reaction; restriction mapping; tissue /cell culture; transfection /expression vector; vitamin antagonist; vitamin metabolism

DESCRIPTION: This project is focused upon determining the molecular genetic basis for alterations in transport that render cells resistant to antifolates by preventing drug accumulation. A full length cDNA will be cloned by preparing an expression library from the Chinese hamster lung cell line DC- 3F/FA3 that appears to overexpress a gene for a 43.2 kd glycoprotein associated with enhanced folate uptake. Functional interactions of this and the hp58 gene product will be studied and used to isolate putative mutant transporters from existing cell lines.

描述：本项目的重点是确定分子