C/EBP ALPHA AS MEDIATOR OF INSULIN ACTION IN ADIPOCYTES

C/EBP ALPHA 作为脂肪细胞中胰岛素作用的调节剂

• 批准号: 2734222
• 负责人: Ormond A MacDougald
• 金额: $ 15.25万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-25 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2734222
• 关键词: adipocytes; affinity chromatography; cell differentiation; chimeric proteins; confocal scanning microscopy; dimer; enhancer binding protein; gel mobility shift assay; gene expression; genetic transcription; hormone regulation /control mechanism; insulin; laboratory rat; mutant; northern blottings; phosphorylation; protein degradation; protein kinase; protein purification; protein sequence; protein structure function; reporter genes; site directed mutagenesis; tissue /cell culture

DESCRIPTION (Adapted from the applicant's abstract): The long-term goal of this project is to further the understanding of the role of the transcription factor C/EBPalpha in regulating adipocyte gene expression and metabolism. C/EBPalpha is induced during the differentiation of 3T3-L preadipocytes into adipocytes and activates the expression of genes involved in creating and maintaining the adipocyte phenotype, notably the insulin responsive glucose transporter Glut-4, adipocyte lipid binding protein 422/AP2, and stearoyl CoA desaturase 1 (SCD1) genes. The PI will test the effects of insulin-induced changes in phosphorylation on the activity of C/EBPalpha and elucidate the role of C/EBPalpha in regulating the expression of downstream adipocyte genes.

描述（改编自申请人的摘要）：长期目标 该项目旨在进一步了解 转录因子C/EBPalpha对脂肪细胞基因表达的调控 新陈代谢. C/EBPalpha在3 T3-L的分化过程中被诱导 前脂肪细胞转化为脂肪细胞并激活相关基因的表达 在产生和维持脂肪细胞表型，特别是胰岛素 响应性葡萄糖转运蛋白Glut-4，脂肪细胞脂质结合蛋白 422/AP 2和硬脂酰CoA去饱和酶1（SCD 1）基因。 PI将检测胰岛素诱导的磷酸化变化的影响 对C/EBPalpha活性的影响，并阐明C/EBPalpha在 调节下游脂肪细胞基因的表达。