MAPPING MOUSE LOCI THAT MODIFY CPK-INDUCED PKD

绘制改变 CPK 诱导的 PKD 的小鼠基因座

• 批准号: 2770563
• 负责人: Lisa M Guay-Woodford
• 金额: $ 13.19万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2770563
• 关键词: bile ducts; biliary tract disorder; cell differentiation; disease /disorder model; gene expression; gene mutation; genetic mapping; genetic markers; kidney; laboratory mouse; liver; molecular pathology; northern blottings; phenotype; polycystic kidney; polymerase chain reaction; renal tubule

A major focus in polycystic kidney disease (PKD) research is to identify genes involved in renal cyst development. Despite recent successes in cloning several human PKD genes, their roles in disease pathogenesis remain undefined. In addition, it appears that genetic background influences the expression of many PKD disease-susceptibility genes. The characterization of these putative modifying genes will be quite difficult in the complex, randomly mating, human population. As an alternative, mouse PKD mutations and their genetic modifiers may provide powerful resources to study genes and gene interactions involved in renal cystogenesis. Among the several mouse models in which PKD segregates as a single Mendelian trait, three mutations, cpk, bpk, and Tg737Rpw, closely resemble human autosomal recessive polycystic kidney disease (ARPKD). In these three mouse models as well as human ARPKD, renal cyst formation begins in utero and genetic background influences disease expression. Therefore, we hypothesize that these mammalian genes and their modifiers define a molecular pathway that is important in both renal cyst development and renal tubular differentiation. In this proposal, we will identify and characterize genes that influence the expression of the cpk mutation. Specifically, we will identify genes that accelerate the-development of renal cystic disease and cause bile duct plate abnormalities in the F2 affected progeny of an intersubspecific intercross between C57BL/6J-cpk/+ and Mus mus castaneus. In addition, we will map the bpk mutation, a second mouse model of human ARPKD. Then in a directed fashion, we will test whether the cpk genetic modifiers influence the phenotypic expression of the bpk mutation. Finally, we will construct congenic strains which isolate individual modifying loci in specific genetic backgrounds as the prelude to identifying and characterizing these genes. Therefore, this project will establish the molecular framework for identifying gene(s) that modify the disease pathogenesis in mouse recessive PKD. Our ultimate goal Is to use these genetic tools to dissect the molecular pathogenesis of human ARPKD.

多囊肾病（PKD）研究的一个主要焦点是鉴别

项目成果

Overview: the genetics of renal disease.

概述：肾脏疾病的遗传学。

• 发表时间: 1999
• 期刊: Seminars in nephrology.
• 作者: Guay-Woodford,LM

Diffuse renal cystic disease in children: morphologic and genetic correlations.

儿童弥漫性肾囊性病：形态学和遗传相关性。

• DOI: 10.1007/s004670050431
• 发表时间: 1998
• 期刊: Pediatric nephrology (Berlin, Germany)
• 作者: Guay-Woodford,LM;Galliani,CA;Musulman-Mroczek,E;Spear,GS;Guillot,AP;Bernstein,J
• 通讯作者: Bernstein,J

Mapping of mouse alpha 1(XIII) collagen to chromosome 10 and its exclusion as a kd candidate gene.

小鼠 α 1(XIII) 胶原蛋白到 10 号染色体的定位及其作为 kd 候选基因的排除。

• DOI: 10.1023/a:1002013218535
• 发表时间: 2000
• 期刊: Biochemical genetics
• 影响因子: 2.4
• 作者: Mrug,M;Stockwin,J;Wüthrich,RP;Gasser,DL;Guay-Woodford,LM
• 通讯作者: Guay-Woodford,LM

Prenatal diagnosis of autosomal recessive polycystic kidney disease (ARPKD):: Molecular genetics, clinical experience, and fetal morphology

• DOI: 10.1002/(sici)1096-8628(19980305)76:2<137::aid-ajmg6>3.0.co;2-q
• 发表时间: 1998-03-05
• 期刊: AMERICAN JOURNAL OF MEDICAL GENETICS
• 作者: Zerres, K;Mücher, G;Guay-Woodford, LM
• 通讯作者: Guay-Woodford, LM

Phenotypic variability in PKD1: the family as a starting point.

PKD1 的表型变异：以家族为起点。

• DOI: 10.1046/j.1523-1755.1999.00552.x
• 发表时间: 1999
• 期刊: Kidney international
• 影响因子: 19.6
• 作者: Guay-Woodford,LM