MECHANICS OF THE NCD MICROTUBULE MOTOR PROTEIN

NCD 微管运动蛋白的机制

• 批准号: 2873879
• 负责人: RUSSELL J STEWART
• 金额: $ 10万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-25 至 2000-09-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2873879
• 关键词: biomechanics; genetic manipulation; interferometry; kinesin; lasers; microtubule associated protein; molecular biology; protein binding; protein structure function

The broad objectives of this proposal are to investigate the mechanistic basis for microtubule movement and direction of microtubule movement by kinesin-like microtubule motor proteins, particularly the ncd protein. The strategy will be to compare and contrast molecular details of ncd structure and motility with kinesin structure and motility. The specific aims of the proposal are to conduct quantitative single- molecule motility experiments with ncd and kinesin under identical experimental conditions. A multiple-beam optical tweezer-based instrument capable of nanometer spatial resolution and piconewton-scale force measurements will be used to determine for ncd and kinesin the magnitude and kinetics of single translocation on events, the force profile during the translocation cycle, and the path over the microtubule surface. Precise measurement of the mechanical parameters of ncd and kinesin will be used to establish a basis for genetic manipulations aimed at ncd and kinesin.

本提案的总体目标是调查其机制 微管运动的基础和方向 驱动蛋白样微管马达蛋白，特别是NCD蛋白。 策略是比较和对比NCD的分子细节 结构和运动与驱动蛋白结构和运动。 的 该提案的具体目标是进行量化的单一- 相同条件下NCD和驱动蛋白的分子运动实验 实验条件 一种基于多光束光镊的 一种具有纳米空间分辨率和皮牛顿尺度仪器 力测量将用于确定NCD和驱动力， 单易位的大小和动力学对事件，力 在易位周期中的概况，以及在易位周期中的路径。 微管表面 机械参数的精确测量 NCD和驱动蛋白将被用来建立一个遗传基础， 针对NCD和驱动蛋白的操作。