MECHANICS OF THE NCD MICROTUBULE MOTOR PROTEIN

这项提案的主要目标是研究 微管运动的基础和微管运动的方向 激动素样微管马达蛋白，尤其是NCD蛋白。 策略将是比较和对比非传染性疾病的分子细节 具有动蛋白结构和运动性的结构和运动性。这个 该建议的具体目标是进行量化的单一- NCD和Kinesin在相同条件下的分子运动学实验 实验条件。一种基于多波束的光镊子系统 具有纳米级空间分辨率和皮牛级分辨率的仪器 将使用力测量来确定NCD和运动在 事件中单次移位的大小和动力学，力 在移位周期中的配置文件，以及 微管表面。机械参数的精确测量 NCD和激动素将被用来建立遗传基础 针对非传染性疾病和激动素的手法。

The broad objectives of this proposal are to investigate the mechanistic basis for microtubule movement and direction of microtubule movement by kinesin-like microtubule motor proteins, particularly the ncd protein. The strategy will be to compare and contrast molecular details of ncd structure and motility with kinesin structure and motility. The specific aims of the proposal are to conduct quantitative single- molecule motility experiments with ncd and kinesin under identical experimental conditions. A multiple-beam optical tweezer-based instrument capable of nanometer spatial resolution and piconewton-scale force measurements will be used to determine for ncd and kinesin the magnitude and kinetics of single translocation on events, the force profile during the translocation cycle, and the path over the microtubule surface. Precise measurement of the mechanical parameters of ncd and kinesin will be used to establish a basis for genetic manipulations aimed at ncd and kinesin.