Genetic Manipulation of Retinal Ganglion Cell Subtypes
视网膜神经节细胞亚型的基因操作
基本信息
- 批准号:10688275
- 负责人:
- 金额:$ 20万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-01 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:AblationAddressAnimal BehaviorAnimalsArousalBehaviorBehavioralBrainBrain regionCandidate Disease GeneCell Culture TechniquesCell physiologyCellsCommunitiesComplementConsciousContrast SensitivityCustomDataDevelopmentDyesExploratory/Developmental GrantFluorescent in Situ HybridizationFutureGene ExpressionGenerationsGenesGeneticGenetic MarkersGoalsHealthHormonesHumanImmunohistochemistryIndividualInvestmentsLabelLearningLightLinkMapsMental HealthMiosis disorderModelingModernizationMolecularMoodsMorphologyMusNeuronsNeurosciencesOrganismOutputPhysiologicalPhysiologyPopulationPropertyReagentResearch PersonnelResolutionRetinaRetinal Ganglion CellsRoleSchemeSpecificityStimulusSubconsciousSystemTimeValidationViral VectorVisualVisual FieldsVisual PerceptionVisual Systemadeno-associated viral vectorcandidate identificationcandidate validationcell typecircadianexperiencefrontiergenetic manipulationinsightmelanopsinmolecular markerphysical conditioningresponsesensory systemtooltranscriptome sequencingvisual neurosciencevisual stimulus
项目摘要
PROJECT SUMMARY
Light is a profoundly important regulator of physiology and behavior across a wide range of organisms. Light
information is relayed via diverse retinal ganglion cell types to approximately 50 distinct targets in the brain. The
melanopsin-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs) represent 6 of the 40 retinal
ganglion cell types present in the mouse retina. The M1-M6 ipRGC subtypes each possess a distinct complement
of morphological and physiological features, central projections, and behavioral roles. Thus, mapping of ipRGC
circuits with single subtype resolution would provide critical insight into the function of multiple and varied visual
circuits. However, progress on this front has been hampered by a dearth of genetic tools for label, manipulation,
and ablation of single subtypes of retinal ganglion cell. Our preliminary data indicate that a Cre and Flp-based
intersectional approach could allow for such manipulation of single ipRGC subtypes. This proposal aims to
identify genetic marker combinations unique to single ipRGC subtypes. Additionally, we will simultaneously
create a modular genetic toolkit that can be integrated into any Cre/Flp motif for future ipRGC subtype
manipulation. These tools will not only provide immediate insight into ipRGC-connected circuits, but will provide
critical tools and an experimental pipeline for the visual and circuit neuroscience communities to study a broad
range of circuits to link cellular function to behavior.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Tiffany M. Schmidt其他文献
Tiffany M. Schmidt的其他文献
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{{ truncateString('Tiffany M. Schmidt', 18)}}的其他基金
Genetic Manipulation of Retinal Ganglion Cell Subtypes
视网膜神经节细胞亚型的基因操作
- 批准号:
10528207 - 财政年份:2022
- 资助金额:
$ 20万 - 项目类别:
Non-canonical GABAergic Pathways in the Visual System
视觉系统中的非典型 GABA 能通路
- 批准号:
10443588 - 财政年份:2020
- 资助金额:
$ 20万 - 项目类别:
Non-canonical GABAergic Pathways in the Visual System
视觉系统中的非典型 GABA 能通路
- 批准号:
10630120 - 财政年份:2020
- 资助金额:
$ 20万 - 项目类别:
Non-canonical GABAergic Pathways in the Visual System
视觉系统中的非典型 GABA 能通路
- 批准号:
10202616 - 财政年份:2020
- 资助金额:
$ 20万 - 项目类别:
Administrative Supplement - Non-canonical GABAergic Pathways in the Visual System
行政增补 - 视觉系统中的非规范 GABA 能通路
- 批准号:
10324253 - 财政年份:2020
- 资助金额:
$ 20万 - 项目类别:
Administrative Supplement for R01 EY 030565-01A1 - Contextual Fear Conditioning Apparatus
R01 EY 030565-01A1 的行政补充 - 情境恐惧调节装置
- 批准号:
10661426 - 财政年份:2020
- 资助金额:
$ 20万 - 项目类别:
Developmental origins of intrinsically photosensitive retinal ganglion cells
本质光敏视网膜神经节细胞的发育起源
- 批准号:
8316636 - 财政年份:2012
- 资助金额:
$ 20万 - 项目类别:
Developmental origins of intrinsically photosensitive retinal ganglion cells
本质光敏视网膜神经节细胞的发育起源
- 批准号:
8507004 - 财政年份:2012
- 资助金额:
$ 20万 - 项目类别:
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