DNA REPLICATION AND GENE EXPRESSION OF CHLORELLA VIRUSES

描述：小球藻病毒PBCV-1是一个独特的家族的原型 感染绿藻的大型DNA病毒。这些生物的大基因组 病毒编码了大量的基因，其预测的产物与 包括原核蛋白和真核蛋白。这次更新的主要重点是 建议对小球藻病毒糖基化进行研究。它是 认为PBCV-1可能编码其自身的部分或全部糖基化 机器，这种机器可能不是在ER或高尔基体中运行，而是在ER或高尔基体中运行 在病毒组装中心内。因此，私家侦探建议确定 参与指导糖基化的基因，以确定 PBCV-1糖蛋白上的寡糖，并测定细胞内 糖基化的部位。 这笔赠款的第二个也是次要的目的是追求对 可能含有病变的小球藻病毒耐药突变株 病毒编码的DNA拓扑异构酶II和迷你K+离子通道。是这样的 突变体在结构-功能分析中可能很重要，在提供 一种检测这些蛋白质的新抑制物的方法，这将与 心脏病和癌症的治疗。

DESCRIPTION: Chlorella virus PBCV-1 is the prototype of a unique family of large DNA viruses which infect green algae. The large genomes of these viruses encode a vast repertoire of genes whose predicted products resemble both procaryotic and eucaryotic proteins. The major focus of this renewal proposal is an investigation of Chlorella virus glycosylation. It is thought that PBCV-1 may encode part or all of its own glycosylation machinery, and that this machinery may function not in the ER or Golgi but within viral assembly centers. Thus, the P.I. proposes to identify the genes involved in directing glycosylation, to determine the structure of the oligosacchardie on PBCV-1 glycoproteins, and determine the intracellular site of glycosylation. A second and minor aim of this grant is to pursue the characterization of drug-resistant mutants of Chlorella virus that may contain lesions within the virally encoded DNA topoisomerase II and and mini K+ ion channel. Such mutants could be important in structure-function analyses and in providing an assay for new inhibitors of these proteins, which would bear on treatments for heart disease and cancer.