DNA REPLICATION AND GENE EXPRESSION OF CHLORELLA VIRUSES

描述：小球藻病毒PBCV-1是一个独特的家族的原型， 感染绿色藻类的大型DNA病毒。 它们的大型基因组 病毒编码大量基因，其预测产物类似于 包括原核和真核蛋白。 这次更新的主要重点是 建议是调查小球藻病毒糖基化。 是 认为PBCV-1可能编码其自身的部分或全部糖基化 机器，而且这种机器可能不在ER或高尔基体中发挥作用， 在病毒装配中心。 因此，PI。建议确定 参与指导糖基化的基因，以确定糖基化的结构。 寡糖对PBCV-1糖蛋白的作用，并测定细胞内 糖基化位点。 这项赠款的第二个次要目标是对下列方面进行定性： 小球藻病毒的耐药突变体，可能含有病变内 病毒编码的DNA拓扑异构酶II和迷你K+离子通道。 等 突变体在结构-功能分析和提供 一项针对这些蛋白质的新抑制剂的试验， 心脏病和癌症的治疗方法。

DESCRIPTION: Chlorella virus PBCV-1 is the prototype of a unique family of large DNA viruses which infect green algae. The large genomes of these viruses encode a vast repertoire of genes whose predicted products resemble both procaryotic and eucaryotic proteins. The major focus of this renewal proposal is an investigation of Chlorella virus glycosylation. It is thought that PBCV-1 may encode part or all of its own glycosylation machinery, and that this machinery may function not in the ER or Golgi but within viral assembly centers. Thus, the P.I. proposes to identify the genes involved in directing glycosylation, to determine the structure of the oligosacchardie on PBCV-1 glycoproteins, and determine the intracellular site of glycosylation. A second and minor aim of this grant is to pursue the characterization of drug-resistant mutants of Chlorella virus that may contain lesions within the virally encoded DNA topoisomerase II and and mini K+ ion channel. Such mutants could be important in structure-function analyses and in providing an assay for new inhibitors of these proteins, which would bear on treatments for heart disease and cancer.