DNA REPLICATION AND GENE EXPRESSION OF CHLORELLA VIRUSES

小球藻病毒的 DNA 复制和基因表达

• 批准号: 2405927
• 负责人: JAMES L VAN ETTEN
• 金额: $ 22.54万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-28 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2405927
• 关键词: Chlorophyta; DNA replication; DNA topoisomerases; DNA virus; Escherichia coli; drug resistance; electroporation; endonuclease; enzyme inhibitors; gene expression; gene mutation; gene targeting; glycosylation; glycosyltransferase; host organism interaction; methyltransferase; molecular cloning; molecular genetics; plant virus; potassium channel; site directed mutagenesis; virus genetics

DESCRIPTION: Chlorella virus PBCV-1 is the prototype of a unique family of large DNA viruses which infect green algae. The large genomes of these viruses encode a vast repertoire of genes whose predicted products resemble both procaryotic and eucaryotic proteins. The major focus of this renewal proposal is an investigation of Chlorella virus glycosylation. It is thought that PBCV-1 may encode part or all of its own glycosylation machinery, and that this machinery may function not in the ER or Golgi but within viral assembly centers. Thus, the P.I. proposes to identify the genes involved in directing glycosylation, to determine the structure of the oligosacchardie on PBCV-1 glycoproteins, and determine the intracellular site of glycosylation. A second and minor aim of this grant is to pursue the characterization of drug-resistant mutants of Chlorella virus that may contain lesions within the virally encoded DNA topoisomerase II and and mini K+ ion channel. Such mutants could be important in structure-function analyses and in providing an assay for new inhibitors of these proteins, which would bear on treatments for heart disease and cancer.

描述：小球藻病毒PBCV-1是一个独特的小球藻家族的原型