SURFACTANT PROTEIN-A AND LUNG DEFENSE

描述（根据申请人的摘要和具体目标改编）： 申请旨在确定的作用和机制， 表面活性蛋白-A（SP-A）保护肺免受细菌感染。 SP-A是一种在出生后大量产生的表面活性物质相关蛋白 主要由两个细胞隔室，远端细支气管上皮 以及肺泡II型细胞和气管/支气管腺。 其 结构和功能相似的集合和在体外 作为细菌病原体调理素的性质强烈推断SP-A是 肺的先天免疫系统的重要组成部分。 这 应用，将测试SP-A在体内保护肺的假设 从细菌感染，并减少脓毒症从肺部传播， 增强肺泡巨噬细胞的细菌摄取和杀灭， 外周单核细胞 具体目标涉及：1）作用和机制 SP-A在肺部感染和预防脓毒性传播的细菌; 2)细胞位点和SP-A合成量，以保护 肺免受细菌感染;以及SP-A -/-小鼠肺的保护 用SP-A经气管置换的感染。

DESCRIPTION (Adapted from applicant's abstract and specific aims): This application seeks to determine the role and the mechanism by which surfactant protein-A (SP-A) protects the lung from bacterial infection. SP-A is an abundant surfactant associated protein produced postnatally principally by two cellular compartments, the distal bronchiolar epithelium and alveolar Type II cells and the tracheal/bronchial glands. Its structural and functional resemblance to collections and the in vitro properties as an opsonin of bacterial pathogens strongly infer that SP-A is an important component of the innate immune system of the lung. This application, will test the hypothesis that SP-A protects the lung in vivo from bacterial infection and reduces septic spread from the lung by enhancing bacterial uptake and killing by alveolar macrophages and peripheral monocytes. The specific aims address: 1) the role and mechanism of SP-A during lung infection and prevention of septic spread of bacteria; 2) cellular sites and quantity of SP-A synthesis required to protect the lungs from bacterial infection; and protection of the lung of SP-A -/- mice from infection by transtracheal replacement with SP-A.