SURFACTANT PROTEIN-A AND LUNG DEFENSE

表面活性蛋白 A 和肺部防御

• 批准号: 6183308
• 负责人: Thomas R Korfhagen
• 金额: $ 28.28万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6183308
• 关键词: alveolar macrophages; bacteria infection mechanism; bactericidal immunity; cytokine; free radical oxygen; genetic strain; genetically modified animals; host organism interaction; laboratory mouse; opsonin; protein localization; pulmonary surfactants; respiratory infections

DESCRIPTION (Adapted from applicant's abstract and specific aims): This application seeks to determine the role and the mechanism by which surfactant protein-A (SP-A) protects the lung from bacterial infection. SP-A is an abundant surfactant associated protein produced postnatally principally by two cellular compartments, the distal bronchiolar epithelium and alveolar Type II cells and the tracheal/bronchial glands. Its structural and functional resemblance to collections and the in vitro properties as an opsonin of bacterial pathogens strongly infer that SP-A is an important component of the innate immune system of the lung. This application, will test the hypothesis that SP-A protects the lung in vivo from bacterial infection and reduces septic spread from the lung by enhancing bacterial uptake and killing by alveolar macrophages and peripheral monocytes. The specific aims address: 1) the role and mechanism of SP-A during lung infection and prevention of septic spread of bacteria; 2) cellular sites and quantity of SP-A synthesis required to protect the lungs from bacterial infection; and protection of the lung of SP-A -/- mice from infection by transtracheal replacement with SP-A.

描述（改编自申请人的摘要和特定目的）： 应用程序旨在确定角色和机制 表面活性剂蛋白A（SP-A）可保护肺免受细菌感染。 SP-A是产后产生的丰富表面活性剂相关蛋白 主要是通过两个细胞室，远端支气管上皮 和牙槽II型细胞和气管/支气管。 它是 结构和功能相似之处与收集和体外 作为细菌病原体的opsonin的特性强烈推断出SP-A为 肺先天免疫系统的重要组成部分。 这 应用将检验SP-A保护肺体内肺的假设 从细菌感染中减少脓毒症从肺部传播 增强细菌的摄取和杀死肺泡巨噬细胞的杀伤和 周围单核细胞。 特定目的地址：1）角色和机制 肺部感染期间的SP-A的细菌败血症; 2）保护该保护所需的细胞位点和SP-A合成数量 细菌感染的肺；和保护SP-A - / - 小鼠的肺 从替换sp-a的替换中感染。