TRANSCRIPTIONAL REPRESSION IN EARLY DROSOPHILA EMBRYOS

早期果蝇胚胎的转录抑制

• 批准号: 2774755
• 负责人: Hailan Zhang
• 金额: $ 3.17万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-08 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2774755
• 关键词: Drosophilidae; chimeric proteins; developmental genetics; gene induction /repression; gene mutation; gene targeting; genetic transcription; immunoprecipitation; invertebrate embryology

Transcriptional repression has been implicated in normal biological processes (e.g. animal embryogenesis) and pathological processes (e.g. tumorigenesis). However, the mechanisms of transcriptional repression are still poorly understood. The overall goal of the proposed research is to determine whether short-range and long-range repression represent distinct mechanisms of transcriptional repression in the early Drosophila embryo. Most of the experiments will be conducted in the context of a simple model, whereby the newly identified dCtBP corepressor protein mediates short-range repression, while the Groucho corepressor mediates long-range repression. The research plan includes two specific aims: (i) characterization of Snail-mediated repression by misexpressing Snail and the mutated derivatives lacking the dCtBP interaction motifs (P-DLS-K/R) and by identifying corepressor proteins; and (ii) characterization of Hairy-mediated repression, by misexpressing Gal4-Halry, Snail-Hairy and the mutated Hairy derivatives lacking the dCtBP interaction motif (P-SLV-K) or Groucho interaction motif (WRPW), to determine whether Hairy functions either as a short- range or long-range repressor, respectively.

转录抑制与正常生物过程（例如动物胚胎发生）和病理过程（例如肿瘤发生）有关。 然而，转录抑制的机制仍然知之甚少。该研究的总体目标是确定短程和长程抑制是否代表早期果蝇胚胎中转录抑制的不同机制。大多数实验将在一个简单模型的背景下进行，其中新鉴定的 dCtBP 辅阻遏蛋白介导短程抑制，而 Groucho 辅阻遏蛋白介导长程抑制。 该研究计划包括两个具体目标：(i) 通过错误表达 Snail 和缺乏 dCtBP 相互作用基序 (P-DLS-K/R) 的突变衍生物以及识别辅阻遏蛋白来表征 Snail 介导的抑制； (ii) 通过错误表达 Gal4-Halry、Snail-Hairy 和缺乏 dCtBP 相互作用基序 (P-SLV-K) 或 Groucho 相互作用基序 (WRPW) 的突变 Hairy 衍生物来表征 Hairy 介导的抑制，以确定 Hairy 是否分别作为短程或长程抑制子发挥作用。