EUKARYOTIC LIFE IN ALKALINE AND ACIDIC ENVIRONMENTS

碱性和酸性环境中的真核生命

• 批准号: 6018387
• 负责人: LINDA A AMARAL ZETTLER
• 金额: $ 3.67万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6018387
• 关键词: Protista; acid base balance; acidity /alkalinity; animal genetic material tag; antiport; aquatic organism; biochemical evolution; eukaryote; genetic polymorphism; membrane transport proteins; microorganism culture; nucleic acid probes; saltwater environment; water sampling /testing

Unicellular eukaryotes (Protista) often inhabit environments of extreme Ph. Such environments occur naturally and result from man-made activities. The diversity and physiology of protists living in such environments has been largely under studied. The proposed work will explore the molecular diversity of protists in extremely acidic and alkaline environments and consider the physiological adaptations which allow survival under pH extremes. This proposal aims to test the hypothesis that very diverse mechanisms regulate internal pH concentration in protists living in alkaline versus acidic pH environments. Comparisons of small-subunit ribosomal RNA sequences will be used to describe the molecular diversity of acidic and alkaline environments. The second component of the proposed work will include establishment of laboratory cultures of representative protists and physiological experiments aimed at distinguishing between different possible mechanisms of internal pH maintenance. The physiological experiments will utilize ion-selective vibrating probe technology to distinguish between different kinds of ion-transport mechanisms. These experiments will identify novel mechanisms of regulation which can then be pursued to a greater extent using molecular biological techniques. The proposed work will serve to broaden our understanding of biology in environments on the threshold of sustaining life.

单细胞真核生物（Protista）经常居住在极端环境中 博士学位自然发生，是由人造活动造成的。 生活在这种环境中的生物的多样性和生理学 在很大程度上被研究。拟议的工作将探索分子 极度酸性和碱性环境中的生物多样性，以及 考虑允许在pH下生存的生理适应 极端。该建议旨在检验非常多样化的假设 机制调节居住在生物中的内部pH浓度 碱性与酸性pH环境。小亚基的比较 核糖体RNA序列将用于描述分子多样性 酸性和碱性环境。提议的第二个组成部分 工作将包括建立代表的实验室文化 原生物和生理实验旨在区分 内部pH维护的不同可能机制。这 生理实验将利用离子选择性振动探针 区分不同种类的离子传输的技术 机制。这些实验将确定调节的新机制 然后，可以使用分子生物学在更大程度上追求它 技术。拟议的工作将有助于扩大我们对 在维持生命的阈值的环境中的生物学。