PHOTOTHERAPY OF ANTIBIOTIC RESISTANT WOUND INFECTIONS

抗生素耐药性伤口感染的光疗

• 批准号: 6074580
• 负责人: MERRILL A. BIEL
• 金额: $ 10.87万
• 依托单位: ADVANCED PHOTODYNAMIC TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-15 至 2000-12-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6074580
• 关键词: Pseudomonadaceae; Staphylococcus aureus; Streptococcus pyogenes; antibiotics; drug resistance; dyes; guinea pigs; histology; lasers; photosensitizing agents; phototherapy; wound infection

Photodynamic therapy (PDT) is a modality that uses a photosensitizing agent and laser light to create oxygen radicals resulting in the destruction of cancer cells and bacteria. As this modality destroys bacteria via a different mechanism than antibiotics, its effectiveness is not altered by bacteria that have antibiotic resistance. Recently, there has been a rapid increase in the strains of antibiotic resistant bacteria with over 90% of Staphylococcus aureus strains being methicillin resistant. These bacteria have resulted in an inability to adequately treat common wound infections with antibiotics causing morbidity and death. PDT destruction of antibiotic resistant Staphylococcus aureus in vivo and antibiotic resistant Streptococcus pyogenes and Pseudomonas aeruginosa in vitro has been demonstrated using methylene blue. The proposed research will evaluate the use of methylene blue with 664 nm laser light as an improved device and dye combination for the efficacious treatment of infected surface wounds. This treatment may ultimately be used to sterilize acutely and chronically infected wounds unresponsive to antibiotics. PROPOSED COMMERCIAL APPLICATIONS: The commercial applications of this research is the use of PDT antimicrobial treatment with methylene blue and 664 nm laser light for the sterilization of antibiotic resistant acutely and chronically infected wounds found in debilitated and diabetic patients.

光动力疗法（PDT）是一种使用光敏剂和激光来产生氧自由基，从而破坏癌细胞和细菌的方式。由于这种方式通过与抗生素不同的机制破坏细菌，因此其有效性不会被具有抗生素耐药性的细菌改变。最近，抗生素耐药细菌的菌株迅速增加，超过90%的金黄色葡萄球菌菌株是甲氧西林耐药的。这些细菌导致抗生素无法充分治疗常见的伤口感染，导致发病和死亡。PDT破坏抗生素耐药金黄色葡萄球菌在体内和抗生素耐药酿脓链球菌和铜绿假单胞菌在体外已被证明使用亚甲蓝。拟议的研究将评估使用亚甲蓝与664 nm激光作为有效治疗感染表面伤口的改进器械和染料组合。这种治疗方法最终可能用于对抗生素无反应的急性和慢性感染伤口进行消毒。拟议的商业应用：这项研究的商业应用是使用亚甲基蓝和664 nm激光进行PDT抗菌治疗，用于对虚弱和糖尿病患者中发现的抗生素耐药性急性和慢性感染伤口进行灭菌。