DETECTION OF MACROMOLECULES WITH QUANTUM DYES

描述(逐字摘自申请者摘要)：的长期目标 本项目是发光生物标记物的开发，包含 稀土(III)络合物在大环配体“量子染料”上官能化。 前人的工作表明，官能化的Eu(III)大环配合物 能够共价连接到生物物质上，并允许背景 自由、时间门控发光测量。然而，对于许多实际的人来说 应用时，它们的发光强度必须增加。第一阶段的工作 结果：1)合成了含有多个Eu-大环的标记，2) 添加剂对Eu-大循环发光的增强作用 能量转移供体或提供防止猝灭的保护，以及3)增强剂 被发现促进了原型的非功能化发光 Tb(III-大循环。Eu(III)和Tb的同构对 (Iii)功能化发光宏周期将允许灵敏、精确 比较杂交研究。 在第二阶段：1)对细胞凋亡和DNA合成的敏感测试将 已开发，2)包含多个 欧盟-大循环将被最小化，3)功能化的Tb(III)大循环 将被合成和测试。4)将就如何 最大限度地利用Eu(III)和Eu(III)的组合发光 Tb(III)大循环，以及5)对仪器的改进 将制作检测量子染料。 建议的商业应用： 建议的商业应用程序不可用。

DESCRIPTION (Verbatim from the applicant's abstract): The long-term goal of this project is the development of luminescent biomarkers containing lanthanide(III) complexes functionalized on macrocyclic ligands, "Quantum Dyes." Previous work has shown that functionalized europium(III) macrocyclic complexes are capable of covalent attachment to bio-substances and permit background free, time-gated luminescence measurements. However, for many practical applications their luminescence intensity had to be increased. The Phase I work resulted in: 1) The synthesis of markers containing multiple Eu-macrocycles, 2) Enhancement of the Eu-macrocycle luminescence by additives that act as energy-transfer donors or provide protection from quenching, and 3) An enhancer was found that promotes luminescence from the prototype non-functionalized terbium (III-macrocycle. An isomorphous pair of europium (III) and terbium (III) functionalized luminescent macrocycles will permit sensitive, precise comparative hybridization studies. In Phase II: 1) Sensitive tests for apoptosis and DNA synthesis will be developed, 2) The nonspecific binding of markers containing multiple Eu-macrocycles will be minimized, 3) Functionalized terbium(III) macrocycles will be synthesized and tested. 4) Preliminary work will be performed on how to maximize the luminescence from the combined use of europium (III) and terbium(III) macrocycles, and 5) Improvements to the instrumentation for detecting Quantum Dyes will be made. PROPOSED COMMERCIAL APPLICATION: Proposed commercial application not available.