SUSCEPTIBILITY FACTORS IN BLADDER CARCINOGENESIS

N-乙酰转移酶(NAT)和脱乙酰酶的假说 可能在膀胱癌的易感性中起决定性作用 接受检查。为了实现这一目标，我们将首先确定是否有 在体内的乙酰化表型与 培养的人尿路上皮细胞中NAT或脱乙酰酶的水平 体外培养。其次，我们将比较两组数据的相对分布 患有膀胱癌的活跃吸烟者与非吸烟者之间的乙酰化表型 那些没有表现出这种疾病的吸烟者。体内表型 通过测量尿液中的代谢物来进行检测。 5-乙酰氨基-6-甲酰氨基-3-甲基尿嘧啶和1-甲基黄嘌呤 在服用咖啡因之后。

The hypothesis that the N-acetyltransferases (NAT) and deacetylases might play a determinate role in susceptibility to bladder cancer will be examined. Towards this goal, we will determine first whether there is any correlation between in vivo acetylation phenotyping and that of cellular levels of NAT or deacetylases in human urothelium cultures in vitro. Secondly, we will compare the relative distribution of acetylator phenotype among active smokers who have bladder cancer versus those smokers who do not manifest the disease. The in vivo phenotype determinations will be made by measurement of the urinary metabolites 5-acetylamino-6-formylamino-3-methyluracil and 1-methylxanthine following administration of caffeine.