SUSCEPTIBILITY FACTORS IN BLADDER CARCINOGENESIS

膀胱癌发生的易感因素

• 批准号: 6282022
• 负责人: GEORGE T BRYAN
• 金额: $ 2.43万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6282022
• 关键词: acylation; acyltransferase; bladder neoplasm; cancer risk; carcinogenesis; clinical research; enzyme mechanism; human subject; molecular oncology

The hypothesis that the N-acetyltransferases (NAT) and deacetylases might play a determinate role in susceptibility to bladder cancer will be examined. Towards this goal, we will determine first whether there is any correlation between in vivo acetylation phenotyping and that of cellular levels of NAT or deacetylases in human urothelium cultures in vitro. Secondly, we will compare the relative distribution of acetylator phenotype among active smokers who have bladder cancer versus those smokers who do not manifest the disease. The in vivo phenotype determinations will be made by measurement of the urinary metabolites 5-acetylamino-6-formylamino-3-methyluracil and 1-methylxanthine following administration of caffeine.

N-乙酰转移酶（NAT）和脱乙酰酶 可能在膀胱癌的易感性中起决定性作用， 接受检查。 为了实现这一目标，我们将首先确定是否有 体内乙酰化表型与 人尿道上皮培养物中NAT或脱乙酰酶的细胞水平 体外 其次，我们将比较 患有膀胱癌的活跃吸烟者与 那些没有表现出这种疾病的吸烟者。 体内表型 将通过测量尿代谢物进行测定 5-乙酰氨基-6-甲酰氨基-3-甲基尿嘧啶和1-甲基黄嘌呤 服用咖啡因后。