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AUTOMATED SYNTHESIZER UTILIZING TILTED PLATE CENTRIFUGE

使用斜板离心机的自动合成器

基本信息

批准号：
2792663
负责人：
MICHAL LEBL
金额：
$ 9.97万
依托单位：
SPYDER INSTRUMENTS, INC.
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-02-01 至 1999-07-31
项目状态：
已结题

项目摘要

DESCRIPTION (Adapted from the application): The subject of this proposal is the design, construction, and evaluation of a prototype for a new instrument for the semi- and fully automated, parallel solid-phase synthesis of 768 compounds, which will be highly beneficial to drug discovery and other biomedical research. The advantages of this instrument compared to currently available synthesizers are a significantly higher synthesis throughput, as well as a novel technology for the separation of the solid support from reagent and wash solutions, which is one of the key processes in the automation of solid-phase synthesis, by centrifugation. In this instrument, compounds will be synthesized in the wells of microtiter plates in a centrifuge. The plates are slightly tilted towards the center of centrifugation, thus forming pockets in each well during centrifugation, in which the solid support is collected, while the liquid is expelled from the wells. This process is mechanically simpler and more efficient compared to currently used technologies (e.g., vacuum filtration), thus enabling the manufacture of more robust, flexible, yet less expensive instruments. PROPOSED COMMERCIAL APPLICATION: Spyder Instruments' synthesizers based on the tilted plate technology in semi- or fully automated mode of operation, once commercially available, will have an enormous impact on the simplification and acceleration of the parallel solid-phase synthesis of large compound arrays for drug discovery and biomedical research. Tilted plate centrifugation based fluorous-phase synthesizer will be the first instrument for this emerging technology for parallel synthesis. Simple instruments for solid phase extraction will find application in analytical laboratories, and instruments dedicated to plate washing will be applied in biological laboratories.

描述(改编自申请)：本提案的主题是设计、构造和评估新的半自动、全自动、并联式固相检测仪 768个化合物的合成，这将是非常有益的药物发现和其他生物医学研究。与目前可用的仪器相比，该仪器的优势合成器的合成产量也要高得多。作为一种从固体载体中分离的新技术试剂和洗涤液，这是关键的过程之一通过离心法实现固相合成的自动化。在这仪器，化合物将在微滴定井中合成。离心机里的盘子。车牌略微偏向离心机的中心，从而在每口井中形成口袋离心法，收集固体支撑物，而液体从油井中排出。这一过程在机械上更简单并且与当前使用的技术(例如，真空过滤)，从而能够制造更坚固、更灵活的价格较低的仪器。建议的商业应用：基于倾斜板技术的Spyder Instruments合成器在半自动或全自动操作模式下，一旦商业化可用，将对简化和加速大分子化合物的平行固相合成用于药物发现和生物医学研究的阵列。倾斜板基于离心法的氟相合成器将是第一台这一新兴的并行合成技术的仪器。简单固相萃取仪器将在#年得到应用分析实验室和专门用于洗盘的仪器将应用于生物实验室。