NIR FLUORESCENT LABELS FOR RNA SCREENING IN MICROARRAYS

用于微阵列中 RNA 筛选的近红外荧光标签

• 批准号: 2793648
• 负责人: RICHARD H TULLIS
• 金额: $ 9.95万
• 依托单位: SYNGEN RESEARCH
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2793648
• 关键词: RNA; biomedical equipment development; chemical synthesis; fluorescent dye /probe; fungal genetics; gene expression; genetic screening; infrared radiation; nucleic acid hybridization; nucleic acid probes; nucleic acid quantitation /detection; nucleic acid sequence; technology /technique development

Sensitive fluorescent detection of DNA probes is required for applications such as DNA sequencing, in situ hybridization and multi-component arrays for detecting mRNA expression. Such applications are typically limited in sensitivity by autofluorescence of tissues and cells and other environmental components. Thus, there is a continuing need for highly efficient, low background fluorescent dyes in biological research and clinical diagnostics. Phase I is designed to determine the feasibility of using novel far-red and near-infrared (NIR) fluorescent labeled nucleic acids to detect the hybridization of mRNA to gene microarrays. We have chosen yeast genes and their cognate mRNAs as a suitable test system which may also have broad application in research. In Phase II we will synthesize a family of fluorescent dyes is an optimize their use with DNA and RNA probes. We will also develop the IR scanner so that the labeled probes we offer can be effectively used in the field. In Phase II the labeling conditions and use parameters of the labeled probes will be refined market for analysis of gene expression. PROPOSED COMMERCIAL APPLICATION: The near term applications of this technology are in the research market. Novel, reactive near-infrared dyes which can be directly employed to label nucleic acids can be used in fluorescence in situ hybridization (FISH), detecting mRNA expression in microarrays and potentially in labeling DNA sequencing primers.

应用中需要对DNA探针进行灵敏的荧光检测 例如DNA测序、原位杂交和多组分阵列 用于检测mRNA的表达。这类应用程序通常仅限于 组织和细胞及其他组织的自体荧光的敏感性 环境成分。因此，持续存在着高度的需求 高效低本底荧光染料在生物研究中的应用 临床诊断学。第一阶段旨在确定 利用新型远红外和近红外荧光标记的核酸 酸检测mRNA与基因微阵列的杂交。我们有 选择酵母基因及其同源mRNAs作为合适的检测系统 在研究中也可能有广泛的应用。 在第二阶段，我们将合成一系列荧光染料，这是一个优化 它们与DNA和RNA探针一起使用。我们还将开发红外扫描仪，以便 我们提供的标记探针可以在现场有效使用。在……里面 第二阶段标记探针的标记条件和使用参数 将精炼市场用于基因表达分析。 建议的商业应用： 这项技术的近期应用是在研究市场上。 可直接用于标记的新型活性近红外染料 核酸可用于荧光原位杂交(FISH)， 检测微阵列中的mRNA表达并潜在地标记DNA 测序引物。