RAPID AUTOMATABLE DNA HYBRIDIZATION ANALYSIS SYSTEM

快速自动化 DNA 杂交分析系统

• 批准号: 3505091
• 负责人: RICHARD H TULLIS
• 金额: $ 5万
• 依托单位: IMMUNE COMPLEX CORPORATION
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1986-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3505091
• 关键词: communicable disease diagnosis; enzyme substrate; fluorescent dye /probe; high performance liquid chromatography; rapid diagnosis

DNA and RNA hybridization probes, for use in the clinical diagnosis of infectious disease agents and for genetic screening, are under intensive commercial development. Hybridization probes, either chemically synthesized or derived from natural or recombinant DNA sources, have been shown to be highly specific in the detection and identification of a variety of viruses and bacteria. Synthetic oligonucleotides have also been shown to be useful in detecting genetic abnormalities in a Southern blot format. However, nearly all current methods for the use of hybridization probes require extensive sample preparation, followed by gel-electrophoresis or immobilization on filters, hybridization for up to 24 hours and protracted secondary procedures for detection. These procedures are tedious, time consuming and highly specialized techniques which are not easily automated. In addition, the most senitive detection techniques rely on radioactivity which presents both handling and disposal problems in the clinical lab. There is a need for an efficient, rapid, easily automatable hybridization analysis system which can be applied to relatively crude samples and is compatible with current non-isotopic detection methodologies.

DNA和RNA杂交探针，用于临床诊断 传染病病原体和遗传筛查，正在加紧进行 商业发展。 杂交探针，或者化学杂交， 合成或衍生自天然或重组DNA来源，已经被 在检测和鉴定一种 各种病毒和细菌。 合成的寡核苷酸也已被用于 在Southern印迹检测遗传异常中显示出有用 格式. 然而，几乎所有目前使用的杂交方法都是不稳定的。 探针需要大量的样品制备， 凝胶电泳或固定在过滤器上， 24小时和延长的二次检测程序。 这些 程序是繁琐、耗时和高度专业化的技术 这不容易自动化。 此外，最灵敏的检测 技术依赖于放射性， 临床实验室的问题。 需要一种高效、快速、 一种易于自动化的杂交分析系统， 相对粗糙的样本并且与当前的非同位素兼容 检测方法。