RAPID AUTOMATABLE DNA HYBRIDIZATION ANALYSIS SYSTEM
RAPID AUTOMATABLE DNA HYBRIDIZATION ANALYSIS SYSTEM
批准号:
3505091
负责人:
RICHARD H TULLIS
金额:
$5.0万
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-04-01 至 1986-09-30
中文摘要
DNA和RNA杂交探针在临床诊断中的应用
传染病试剂和基因筛查正在加紧进行中
商业发展。杂交探针,化学上的
从天然或重组DNA来源合成或衍生的,已被
显示出在检测和识别一种
各种病毒和细菌。人工合成的寡核苷酸也已经被
被证明在检测Southern杂交中的遗传异常是有用的
格式化。然而,目前使用的几乎所有杂交方法
探头需要大量的样品准备,其次是
凝胶电泳法或固定化滤网,杂交,最高可达
24小时和长时间的二次检测程序。这些
程序是繁琐、耗时和高度专业化的技术
这并不容易实现自动化。此外,最灵敏的检测
技术依赖于放射性,它既有处理又有处置。
临床实验室的问题。需要一种高效、快速、
易于自动化的杂交分析系统,可应用于
相对原始的样品,与目前的非同位素相容
检测方法。
英文摘要
DNA and RNA hybridization probes, for use in the clinical diagnosis of
infectious disease agents and for genetic screening, are under intensive
commercial development. Hybridization probes, either chemically
synthesized or derived from natural or recombinant DNA sources, have been
shown to be highly specific in the detection and identification of a
variety of viruses and bacteria. Synthetic oligonucleotides have also been
shown to be useful in detecting genetic abnormalities in a Southern blot
format. However, nearly all current methods for the use of hybridization
probes require extensive sample preparation, followed by
gel-electrophoresis or immobilization on filters, hybridization for up to
24 hours and protracted secondary procedures for detection. These
procedures are tedious, time consuming and highly specialized techniques
which are not easily automated. In addition, the most senitive detection
techniques rely on radioactivity which presents both handling and disposal
problems in the clinical lab. There is a need for an efficient, rapid,
easily automatable hybridization analysis system which can be applied to
relatively crude samples and is compatible with current non-isotopic
detection methodologies.
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会议论文
NIR FLUORESCENT LABELS FOR RNA SCREENING IN MICROARRAYS
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批准号:2793648
-
项目类别:
-
资助金额:$9.95万
-
财政年份:1999
-
负责人:RICHARD H TULLIS
-
依托单位:
ENZYMATIC SYNTHESIS OF DEFINED SEQUENCE DNA
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批准号:2859179
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项目类别:
-
资助金额:$9.94万
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财政年份:1998
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负责人:RICHARD H TULLIS
-
依托单位:
AMPHIPHILIC OLIGONUCLEOTIDE CONJUGATES
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批准号:2180216
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项目类别:
-
资助金额:$5.0万
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财政年份:1988
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负责人:RICHARD H TULLIS
-
依托单位:
NEW METHODS FOR SYNTHESIS OF FLUORESCENT DNA
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批准号:3497835
-
项目类别:
-
资助金额:$5.0万
-
财政年份:1987
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负责人:RICHARD H TULLIS
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依托单位:
SYNTHESIS AND USE OF PHOSPHORUS MODIFIED OLIGONUCLEOTIDE
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批准号:3497718
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项目类别:
-
资助金额:$5.0万
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财政年份:1986
-
负责人:RICHARD H TULLIS
-
依托单位:
SYNTHETIC DNA AFFINITY MATRICES FOR HYBRIDIZATION ASSAYS
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批准号:3497541
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项目类别:
-
资助金额:$5.0万
-
财政年份:1985
-
负责人:RICHARD H TULLIS
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依托单位:
海外基金