ACTIN POLYMERIZATION DURING ASCIDIAN SPERM ACTIVATION

描述：这项新的助学金申请包括本科生 肌动蛋白细丝参与了一个重要的， 虽然是独一无二的，但在海鞘中发生了受精事件。那次活动就是 精子过程中线粒体从头到尾的移位 为部分海鞘提供穿透力量的激活 蛋黄皮毛。该提案包括使用数字视频 显微镜，电子显微镜，免疫印迹，免疫荧光， 显微测序、蛋白质纯化方法、单抗 生产、细胞通透性和肌动蛋白探针。具体目标是： 1)研究肌动蛋白聚合的规律和过程。 线粒体易位；2)研究线粒体易位的结构性质 肌动蛋白细丝及其与线粒体和血浆的连接 薄膜。

DESCRIPTION: This new grant application includes undergraduate students in the investigation of the involvement of actin filaments in an important, though unique, fertilization event in ascidians. That event is the mitochondrial translocation occurring from head to tail during sperm activation that provides the force for penetration of part of the ascidian egg vitelline coat. The proposal includes the use of digital video microscopy, electron microscopy, Western blotting, immunofluorescence, microsequencing, protein purification methods, monoclonal antibody production, cell permeabilization and actin probes. The specific aims are: 1) to study the regulation and process of actin polymerization as it relates to mitochondrial translocation; 2) to study the structural nature of the actin filaments and their attachments to the mitochondrion and the plasma membrane.