ACTIN POLYMERIZATION DURING ASCIDIAN SPERM ACTIVATION

海鞘精子激活过程中的肌动蛋白聚合

• 批准号: 2618276
• 负责人: ROBERT A KOCH
• 金额: $ 9.45万
• 依托单位: CALIFORNIA STATE UNIVERSITY FULLERTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2618276
• 关键词: Tunicata; actins; electron microscopy; fertilization; immunofluorescence technique; mitochondria; polymerization; protein purification; protein structure function; sperm; video microscopy; vitelline membrane; western blottings

DESCRIPTION: This new grant application includes undergraduate students in the investigation of the involvement of actin filaments in an important, though unique, fertilization event in ascidians. That event is the mitochondrial translocation occurring from head to tail during sperm activation that provides the force for penetration of part of the ascidian egg vitelline coat. The proposal includes the use of digital video microscopy, electron microscopy, Western blotting, immunofluorescence, microsequencing, protein purification methods, monoclonal antibody production, cell permeabilization and actin probes. The specific aims are: 1) to study the regulation and process of actin polymerization as it relates to mitochondrial translocation; 2) to study the structural nature of the actin filaments and their attachments to the mitochondrion and the plasma membrane.

描述：这个新的补助金申请包括本科生在 肌动蛋白丝参与一个重要的， 海鞘的受精过程虽然独特 这一事件是 精子发生时线粒体从头到尾的易位 为海鞘的一部分的穿透提供力量的激活 卵卵黄层 该提案包括使用数字视频 显微镜，电子显微镜，蛋白质印迹，免疫荧光， 微测序，蛋白纯化方法，单克隆抗体 生产、细胞透化和肌动蛋白探针。 具体目标是： 1)研究肌动蛋白聚合的规律和过程， 线粒体易位; 2）研究线粒体的结构性质， 肌动蛋白丝及其与细胞质和血浆的连接 膜的