APO B KINETICS PRE AND POST TRAINING IN ELDERLY ADULTS

这项研究项目的主要目的是检查影响 运动训练对载脂蛋白B动力学参数的影响 老年人的新陈代谢。血浆低密度水平升高 脂蛋白胆固醇(LDL-C)和载脂蛋白B与一种 增加患冠心病和心肌梗死的风险。在……里面 此外，与年龄相关的血浆低密度脂蛋白-C和载脂蛋白B增加。 浓度。因此，心血管事件的风险增加 在老年人中至少可以部分解释，由年龄相关 对载脂蛋白B代谢的影响。血浆中的载脂蛋白B水平是 它们的生产速度和它们的速度 被分解了。然而，载脂蛋白B代谢的变化发生在 随着年龄的增长一直没有得到很好的描述。 运动训练已被证明可以降低低密度脂蛋白和载脂蛋白B血浆 健康成年人的注意力集中。然而，潜在的机制是 运动诱导的载脂蛋白B浓度的有益变化仍然存在 不清楚。具体地说，这个项目的目的是澄清 运动训练是否减少和/或增加产量 含apoB的脂蛋白分解代谢，从而增强 老年人的心血管风险概况。 在16周运动训练前后，总胆固醇， 甘油三酯、血浆脂蛋白(极低密度脂蛋白、低密度脂蛋白、低密度脂蛋白)和载脂蛋白b 级别将被确定。载脂蛋白B的合成速率将为 通过测量运动训练前后的 静脉注射[1-13C]亮氨酸的参入率 从血浆中分离的极低密度脂蛋白、低密度脂蛋白和低密度脂蛋白的载脂蛋白B部分。血浆和 用气相色谱法测定载脂蛋白B[1-13C]亮氨酸的含量 质谱学，并用它来求解多室模型 载脂蛋白B代谢以前是由副研究员开发的。 该方案的结果将描述运动训练的特点 调节含载脂蛋白和Will的载脂蛋白的合成速率 增加对老年人胆固醇代谢的了解。

The major objective of this research project is to examine the effects of exercise training on kinetic parameters of apolipoprotein B (apoB) metabolism in elderly adults. Elevated levels of plasma low density lipoprotein cholesterol (LDL-C) and apoB have been associated with an increased risk of coronary heart disease and myocardial infarction. In addition, there is an age related increase in plasma LDL-C and apoB concentrations. As such, the increased risk for cardiovascular events in the elderly may be explained at least in part, by the age related effect on apoB metabolism. ApoB levels in plasma are a function of the rate at which they are produced as well as the rate at which they are catabolized. Nevertheless, the changes in apoB metabolism occurring with aging have not been well characterized. Exercise training has been shown to decrease LDL-C and apoB plasma concentration in healthy adults. The underlying mechanism, however, for the exercise-induced salutary changes in apoB concentration remains unclear. Specifically, the purpose of this project is to clarify whether exercise training decreases production and/or increases catabolism of apoB containing lipoproteins, thus enhancing cardiovascular risk profile in elderly adults. Before and after sixteen weeks of exercise training, total cholesterol, triglyceride, plasma lipoprotein (VLDL, IDL, LDL) and apolipoprotein b levels will be determined. The synthesis rate of apoB will be determined before and after exercise training by measuring the incorporation rate of intravenously infused [1-13C] leucine into the apoB fraction of VLDL, IDL and LDL isolated from plasma. Plasma and apoB [1-13C] leucine enrichment will be determined by gas chromatography mass spectrometry, and used to solve the multi compartmental model of apoB metabolism previously developed by the associate investigators. The results of this protocol will characterize how exercise training regulated the synthetic rate of apoB containing lipoproteins and will add to understanding of cholesterol metabolism in the elderly.