APO B KINETICS PRE AND POST TRAINING IN ELDERLY ADULTS

老年人 APO B 动力学训练前后

• 批准号: 2881703
• 负责人: THEODORE J Angelopoulos
• 金额: $ 8.21万
• 依托单位: UNIVERSITY OF CENTRAL FLORIDA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-15 至 2001-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2881703
• 关键词: aging; apolipoprotein B; blood lipoprotein metabolism; chemical kinetics; cholesterol; clinical research; exercise; human old age (65+); human subject; training

The major objective of this research project is to examine the effects of exercise training on kinetic parameters of apolipoprotein B (apoB) metabolism in elderly adults. Elevated levels of plasma low density lipoprotein cholesterol (LDL-C) and apoB have been associated with an increased risk of coronary heart disease and myocardial infarction. In addition, there is an age related increase in plasma LDL-C and apoB concentrations. As such, the increased risk for cardiovascular events in the elderly may be explained at least in part, by the age related effect on apoB metabolism. ApoB levels in plasma are a function of the rate at which they are produced as well as the rate at which they are catabolized. Nevertheless, the changes in apoB metabolism occurring with aging have not been well characterized. Exercise training has been shown to decrease LDL-C and apoB plasma concentration in healthy adults. The underlying mechanism, however, for the exercise-induced salutary changes in apoB concentration remains unclear. Specifically, the purpose of this project is to clarify whether exercise training decreases production and/or increases catabolism of apoB containing lipoproteins, thus enhancing cardiovascular risk profile in elderly adults. Before and after sixteen weeks of exercise training, total cholesterol, triglyceride, plasma lipoprotein (VLDL, IDL, LDL) and apolipoprotein b levels will be determined. The synthesis rate of apoB will be determined before and after exercise training by measuring the incorporation rate of intravenously infused [1-13C] leucine into the apoB fraction of VLDL, IDL and LDL isolated from plasma. Plasma and apoB [1-13C] leucine enrichment will be determined by gas chromatography mass spectrometry, and used to solve the multi compartmental model of apoB metabolism previously developed by the associate investigators. The results of this protocol will characterize how exercise training regulated the synthetic rate of apoB containing lipoproteins and will add to understanding of cholesterol metabolism in the elderly.

本研究项目的主要目的是检验 运动训练对载脂蛋白B（apoB）动力学参数的影响 老年人的新陈代谢。 血浆低密度水平升高 脂蛋白胆固醇（LDL-C）和载脂蛋白B与 冠心病和心肌梗死的风险增加。 在 此外，血浆LDL-C和apoB存在年龄相关性增加 浓度的因此，心血管事件的风险增加 在老年人中，至少可以部分地解释为与年龄相关的 影响apoB代谢。 血浆中的ApoB水平是 它们的生产率以及它们的生产率 分解代谢 尽管如此，apoB代谢发生了变化 还没有很好地表征。 运动训练可降低LDL-C和apoB血浆水平 在健康成年人中的浓度。 然而， 运动引起的apoB浓度的有益变化 不清楚 具体而言，本项目的目的是澄清 运动训练是否会减少产量和/或增加产量 含有载脂蛋白B的脂蛋白的催化剂，从而增强 老年人的心血管风险特征。 在16周的运动训练前后，总胆固醇， 甘油三酯、血浆脂蛋白（VLDL、IDL、LDL）和载脂蛋白B 水平将被确定。 apoB的合成速率将是 通过测量运动训练前后的 静脉输注[1- 13 C]亮氨酸的掺入率 从血浆中分离的VLDL、IDL和LDL的apoB部分。 血浆和 apoB [1- 13 C]亮氨酸富集将通过气相色谱法测定 质谱，并用于解决多房室模型的 载脂蛋白B代谢先前由助理研究者开发。 本方案的结果将描述运动训练如何 调节含apoB脂蛋白的合成速率， 增加对老年人胆固醇代谢的了解。

项目成果

Apolipoprotein E genotype and sex influence C-reactive protein levels regardless of exercise training status.

无论运动训练状态如何，载脂蛋白 E 基因型和性别都会影响 C 反应蛋白水平。

• DOI: 10.1016/j.metabol.2008.04.013
• 发表时间: 2008
• 期刊: Metabolism: clinical and experimental
• 作者: Angelopoulos,TheodoreJ;Miles,MaryP;Lowndes,Joshua;Sivo,StephenA;Seip,RichardL;Pescatello,LindaS;Zoeller,RobertF;Visich,PaulS;Gordon,PaulM;Moyna,NiallM;Thompson,PaulD
• 通讯作者: Thompson,PaulD

Leptin responses to long-term cardiorespiratory exercise training without concomitant weight loss: a prospective study.

• 发表时间: 2008-09
• 期刊: The Journal of sports medicine and physical fitness
• 作者: J. Lowndes;R. Zoeller;J. D. Caplan;G. Kyriazis;N. Moyna;R. Seip;P. Thompson;T. Angelopoulos

Do age and baseline LDL cholesterol levels determine the effect of regular exercise on plasma lipoprotein cholesterol and apolipoprotein B levels?

• DOI: 10.1007/s00421-007-0537-1
• 发表时间: 2007-08
• 期刊: European Journal of Applied Physiology
• 影响因子: 3
• 作者: T. Angelopoulos;S. Sivo;G. Kyriazis;J. D. Caplan;R. Zoeller;J. Lowndes;R. Seip;P. Thompson

Interactive effects of APOE haplotype, sex, and exercise on postheparin plasma lipase activities.

APOE 单倍型、性别和运动对肝素后血浆脂肪酶活性的交互影响。

• DOI: 10.1152/japplphysiol.00287.2010
• 发表时间: 2011
• 期刊: Journal of applied physiology (Bethesda, Md. : 1985)
• 作者: Seip,RichardL;Zoeller,RobertF;Angelopoulos,TheodoreJ;Salonia,James;Bilbie,Cherie;Moyna,NiallM;Miles,MaryP;Visich,PaulS;Pescatello,LindaS;Gordon,PaulM;Tsongalis,GregoryJ;Bausserman,Linda;Thompson,PaulD
• 通讯作者: Thompson,PaulD