ALBUMIN SOLDER FOR WOUND CLOSURE

用于伤口闭合的白蛋白焊料

• 批准号: 2643509
• 负责人: ELIAHU HELDMAN
• 金额: $ 10万
• 依托单位: PANACOS PHARMACEUTICALS, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2643509
• 关键词: acidity /alkalinity; albumins; antiseptic sterilization; bioengineering /biomedical engineering; biotechnology; cell adhesion; cell proliferation; chromatography; drug design /synthesis /production; fibroblasts; guinea pigs; heat; ionic strengths; lasers; lyophilization; method development; protein denaturation; protein engineering; resin; short chain fatty acid; surgery material /equipment; thermostability; tissue /cell culture; tryptophan; wound healing

Our objective is to develop an albumin solder as a complete or partial substitute for sutures or staples during surgery. The solder is applied on the wound and welding is achieved by laser irradiation. The welding requires heat that may cause tissue necrosis. The investigators propose to produce a "low temperature" solder by removing heat stabilizers from albumin. To obtain a product suitable for clinical use, they plan to introduce an additional viral inactivation step based on UVC irradiation, to explore chromatographic techniques appropriate for the removal of heat stabilizers (to reduce denaturation temperature), and to explore a suitable bacterial sterilization technique based on either gamma irradiation or sterile filtration. The methods of evaluation of the solder quality and performance include: (i) quantitative determination of heat stabilizers, (ii) determination of denaturation temperature, (iii) in vitro determination of bonding strength using hairless guinea pig skin, (iv) monitoring wound healing in vivo in a dermal incision model in hairless guinea pigs, and (v) effect on cell cultures relevant to wound healing. The significance of an albumin solder for wound closure stems from limitations of the conventional techniques, such as stapling and suturing, which in certain surgical procedures are difficult and time consuming and may leak or bleed at the suture line. PROPOSED COMMERCIAL APPLICATION: "Low temperature" albumin solder will provide a replacement for sutures and staples. The clinical applications range from micro- to macro- surgery. The albumin solder proposed here will be particularly useful in minimally invasive surgery when sutures and staples are either inappropriate, cumbersome, or time consuming. Albumin solder has a market potential of at least $100 million.

我们的目标是开发一种白蛋白焊料， 在手术中代替缝线或斯台普斯。 焊料应用于 并通过激光照射实现焊接。 焊接 需要加热，这可能导致组织坏死。 研究人员建议， 为了生产“低温”焊料， 白蛋白。 为了获得适合临床使用的产品，他们计划 引入基于UVC额外病毒灭活步骤 辐照，探索适合于 去除热稳定剂（以降低变性温度），以及 探索一种合适的细菌灭菌技术， γ辐射或无菌过滤。 评价方法 焊接质量和性能包括：（i）定量 热稳定剂的测定，（ii）变性的测定 温度，（iii）体外测定粘合强度， 无毛豚鼠皮肤，（iv）在无毛豚鼠皮肤中监测体内伤口愈合， 无毛豚鼠皮肤切口模型，和（v）对细胞的影响 与伤口愈合相关的文化。 白蛋白的重要性 用于伤口闭合的焊料源于传统的 在某些外科手术中， 手术是困难和耗时的，并且可能在 缝合线 拟定商业应用： “低温”白蛋白焊料将替代缝线 还有斯台普斯钉临床应用范围从微观到宏观， 手术这里提出的白蛋白焊料将特别有用 在微创手术中，缝线和斯台普斯 不适当的、麻烦的或耗时的。白蛋白焊料具有 市场潜力至少1亿元。