HIGH SIGNAL/NOISE NONISOTOPIC GENE EXPRESSION ASSAYS

描述（改编自研究者摘要）：申请人 建议开发和利用一种基于特氟隆的膜， 微孔玻璃（CPG）在核酸诊断中的应用。 的优势 膜是其低非特异性结合特性。 这将是 在非同位素测定中利用，由于低的 非特异性背景和低自发荧光。 申请人提出使用该膜来产生“基因定量 包含共价连接至寡核苷酸的寡核苷酸阵列的“卡”， 膜的表面。 这些卡片是为在 临床和研究实验室。 拟定商业应用：不可用

DESCRIPTION (Adapted from investigator's abstract): The applicants propose to develop and exploit a membrane based on Teflon and controlled pore glass (CPG) in nucleic acid diagnostics. The advantage of the membrane is its low nonspecific binding properties. This would be exploited in non-isotopic assays with improved sensitivity due to low nonspecific backgrounds and low autofluorescence. The applicants propose to use the membrane to produce "Gene Quantification Cards" that comprised an array of oligonucleotides covalently linked to the surface of the membrane. The cards are intended for use in the clinical and research laboratory. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE