CORE--BIOLOGICAL AND BIOCHEMICAL SUPPORT

这一核心将为不同的 端粒/端粒酶项目。首先，它将通过以下方式节约资源 防止不必要的常规分析重复，这可能需要 由几个参与小组表演。其次，它将 在实验之间实现更大的一致性，这些实验源于 研究小组的调查。 该核心提供的分析如下： *分离和测定S100提取物和S100提取物中的端粒酶活性 从培养的肿瘤细胞、杂交细胞系和 直接从病人身上取出的肿瘤。 *测试端粒酶活性是否受到各种药物的抑制 是由节目L产生的，节目2。节目3，化学核心， 以及来自外部协作来源(NCI)，使用我们建立的 程序或L项目中提出的快速检测方法。 *评估端粒/端粒酶的生长抑制活性 交互式代理。 *测定端粒长度。 *测定细胞中的双着丝粒染色体数目。 *评估肿瘤细胞的克隆存活情况。

This core will provide two fundamental benefits to the various telomere/telomerase projects. First, it will conserve resources by preventing unnecessary duplication of routine assays that may need to be performed by several of the participating groups. Secondly, it will achieve greater consistency between experiments that arise from the investigations of the research groups. The assays provided by this core are as follows: * To isolate and assay the telomerase activity in S100 extracts and freeze-thaw extracts from cultured tumor cells, hybrid cell lines, and tumors taken directly from patients. * To test whether telomerase activity is inhibited by various agents that are generated from Program l, Program 2. Program 3, the Chemistry Core, and from outside collaborative sources (N.C.I.), using our established procedures or the rapid assays proposed in program l. * To evaluate the growth inhibitory activity of telomere/telomerase interactive agents. * To determine telomere length. * To determine the number of dicentric chromosomes in cells. * To assess the clonogenic survival of tumor cells.