CORE--BIOLOGICAL AND BIOCHEMICAL SUPPORT

这一核心将为不同的国家提供两个基本的好处， 端粒/端粒酶项目。 第一，节约资源， 防止不必要地重复可能需要 由几个参与的团体表演。其次将 在实验之间实现更大的一致性， 研究小组的调查。 该核心提供的检测试剂盒如下： * 分离并测定S100细胞提取物中端粒酶活性， 来自培养的肿瘤细胞、杂交细胞系的冻融提取物， 直接从病人身上取下的肿瘤 * 为了测试端粒酶活性是否被各种试剂抑制， 是从程序1和程序2中产生的。程序3，化学核心， 和外部合作来源（N.C.I.），利用我们现有的 程序或程序L中提出的快速测定。 * 评价端粒/端粒酶的生长抑制活性 互动代理 * 以确定端粒长度。 * 测定细胞中双着丝粒染色体的数目。 * 评估肿瘤细胞的克隆形成存活率。

This core will provide two fundamental benefits to the various telomere/telomerase projects. First, it will conserve resources by preventing unnecessary duplication of routine assays that may need to be performed by several of the participating groups. Secondly, it will achieve greater consistency between experiments that arise from the investigations of the research groups. The assays provided by this core are as follows: * To isolate and assay the telomerase activity in S100 extracts and freeze-thaw extracts from cultured tumor cells, hybrid cell lines, and tumors taken directly from patients. * To test whether telomerase activity is inhibited by various agents that are generated from Program l, Program 2. Program 3, the Chemistry Core, and from outside collaborative sources (N.C.I.), using our established procedures or the rapid assays proposed in program l. * To evaluate the growth inhibitory activity of telomere/telomerase interactive agents. * To determine telomere length. * To determine the number of dicentric chromosomes in cells. * To assess the clonogenic survival of tumor cells.