FACTORS THAT MODIFY INSULIN ACTION

改变胰岛素作用的因素

• 批准号: 2850495
• 负责人: MARIA G BUSE
• 金额: $ 22.76万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-05-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2850495
• 关键词: 3T3 cells; N acetylglucosamine; amidophosphoribosyltransferase; biological signal transduction; carbohydrate biosynthesis; enzyme activity; genetically modified animals; glucose; glucose transport; glucose transporter; glycosylation; hexosamines; immunoprecipitation; insulin; insulin receptor; insulin sensitivity /resistance; intracellular transport; laboratory mouse; laboratory rat; matrix assisted laser desorption ionization; posttranslational modifications; protein kinase C; protein transport; protein tyrosine phosphatase; western blottings

"Glucose toxicity" accounts for insulin resistance in uncontrolled Type I diabetes (IDDM) and contributes to insulin resistance in Type II diabetes (NIDDM). Sustained hyperglycemia or hyperinsulinemia cause insulin resistance; glucose and insulin act synergistically in down- regulating insulin-stimulated glucose transport. A hypothesis to be tested in 3T3-Ll adipocytes is that glucose/insulin induced glucose transport desensitization reflects altered subcellular trafficking of the glucose transporter, GLUT4, which may involve impaired GLUT4 translocation and inappropriate association of GLUT4 containing vesicles (GCV) with the plasma membrane. Products of the hexosamine synthesis pathway (HNSP) have been implicated in glucose-induced insulin resistance; glutamine-fructose-6-P amidotransferase (GFAT) is the rate limiting enzyme and UDP-N-acetyl glucosamine (UDP-GlcNAc) the major product. The role of HNSP will be tested by examining whether conditions which increase or decrease flux via HNSP augment or mitigate, respectively, glucose induced insulin resistance. O-GlcNAcylation is a reversible process, involving O-glycosylation of proteins on Ser/Thr residues with monosaccharide GlcNAc. It usually involves phosphorylation sites and may be regulatory. Based on preliminary data in muscles of a mouse model of insulin resistance, over-expressing GLUTI in muscle, the hypothesis will be tested that increased flux via HNSP promotes O-GlcNAcylation of critical proteins involved in insulin- stimulated glucose transport. These may include GSV-associated proteins, possibly GLUT4 itself and/or proteins associated with GSV docking and fusion. Since adaptive regulation usually involves multiple sites,, we will test the hypothesis that glucose-induced insulin resistance represents in part down-regulation of the insulin receptor (IR) signaling cascade, attempt to identify the major regulatory sites and critically assess the possible contribution of HNSP to the glucose effect. If warranted, the involvement of modulators of IR signal transduction, I.E. protein kinase C (PKC) isoforms, and candidate protein tyrosine phosphatases (PTP-ases: PTP-1B, SH-PTP2 and LAR) will be examined. GFAT activity is allosterically regulated by UDP-GlcNAc, and is modulated in vivo in muscle by the hormonal and metabolic milieu. The pre- and post-translational regulation of GFAT expression will be studied in muscles of rodent models.

“葡萄糖毒性”解释了未控制的I型糖尿病（IDDM）的胰岛素抵抗，并有助于II型糖尿病（NIDDM）的胰岛素抵抗。持续高血糖或高胰岛素血症引起胰岛素抵抗；葡萄糖和胰岛素协同作用，下调胰岛素刺激的葡萄糖运输。在3T3-Ll脂肪细胞中有待验证的一个假设是，葡萄糖/胰岛素诱导的葡萄糖转运脱敏反应了葡萄糖转运蛋白GLUT4亚细胞转运的改变，这可能涉及GLUT4易位受损和含GLUT4囊泡（GCV）与质膜的不适当关联。己糖胺合成途径（HNSP）的产物与葡萄糖诱导的胰岛素抵抗有关；谷氨酰胺-果糖-6- p氨基转移酶（GFAT）是其限速酶，其主要产物是udp - n -乙酰氨基葡萄糖（UDP-GlcNAc）。HNSP的作用将通过检查通过HNSP增加或减少通量的条件是否分别增强或减轻葡萄糖诱导的胰岛素抵抗来测试。o - glcn酰化是一个可逆过程，涉及单糖GlcNAc将丝氨酸/苏氨酸残基上的蛋白质进行o -糖基化。它通常涉及磷酸化位点，可能具有调节作用。根据胰岛素抵抗小鼠肌肉模型的初步数据，肌肉中过度表达GLUTI的假设将得到验证，即通过HNSP增加通量可促进参与胰岛素刺激的葡萄糖运输的关键蛋白的o - glcn酰化。这些可能包括GSV相关蛋白，可能是GLUT4本身和/或与GSV对接和融合相关的蛋白。由于适应性调节通常涉及多个位点，我们将验证葡萄糖诱导的胰岛素抵抗部分代表胰岛素受体（IR）信号级联下调的假设，试图确定主要的调节位点，并批判性地评估HNSP对葡萄糖效应的可能贡献。如果有必要，将检查IR信号转导调节剂的参与，即蛋白激酶C （PKC）异构体和候选蛋白酪氨酸磷酸酶（ptp酶：PTP-1B， SH-PTP2和LAR）。GFAT活性受UDP-GlcNAc的变构调节，在体内肌肉中受激素和代谢环境的调节。我们将在啮齿类动物肌肉模型中研究GFAT表达的翻译前和翻译后调控。