CHLAMYDIA PNEUMONIAE ANTIGENS OF BIOLOGICAL SIGNIFICANCE

具有生物学意义的肺炎衣原体抗原

• 批准号: 2887732
• 负责人: LEE ANN CAMPBELL
• 金额: $ 24.15万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2887732
• 关键词: SDS polyacrylamide gel electrophoresis; antibody specificity; autoradiography; bacterial antigens; bacterial pneumonia; bacterial vaccines; chlamydial disease; communicable disease diagnosis; disease /disorder etiology; enzyme linked immunosorbent assay; human tissue; immunologic assay /test; immunopathology; laboratory mouse; laboratory rabbit; molecular cloning; neutralizing antibody; nucleic acid probes; nucleic acid sequence; serology /serodiagnosis; thin layer chromatography; tissue /cell culture; vaccine development; virulence

Chlamydia pneumoniae is an etiology of acute respiratory diseases in humans. In addition, C.pneumoniae has been associated with chronic respiratory diseases and some diseases of immunopathology. The mechanisms by which C.pneumoniae infections trigger these immunopathologic diseases are largely unknown. The clinical spectrum of C.pneumoniae infection has been extended to atherosclerosis and related clinical manifestations such as coronary heart disease, the number one killer in the U.S. By multiple methods, evidence of the organism has been found in atheromas throughout the arterial tree. If the organism is shown to play a role in atherogenesis, there will be an urgent need to identify vaccine candidates and to develop rapid diagnostic methods. These measures should be aided by elucidation of those factors contributing to C.pneumoniae pathogenesis. There is a paucity of information available on virulence factors of C.pneumoniae and potential targets for prevention of infection. The proposal will seek through molecular, functional, and antigenic analyses to determine the role of following putative virulence factors by: 1) identifying and characterizing the antigens recognized by the C.pneumoniae specific monoclonal antibody because this antibody recognizes surface antigens, has infectivity neutralizing activity, and reacts specifically with C.pneumoniae; 2) identifying the C.pneumoniae antigen which elicits C.pneumoniae specific neutralizing antibodies: a 98-kDa and 42-kDa antigens containing C.pneumoniae specific reactivities recognized during human infection which are also found in circulating immune complexes in persons with coronary artery disease; 3) characterizing a novel genus chlamydial 70-kDa albumin like protein that contains neutralizing epitopes. The hypothesis of antigen mimicry will be addressed by screening sera collected from past studies on immunopathologic diseases associated with chlamydial infection. The ultimate goals of these studies are to elucidating C.pneumoniae antigens contributing to pathogenesis and immunonopathologies that will direct future studies of developing measures for prevention, intervention, and diagnosis.

肺炎衣原体是急性呼吸道疾病的病原体， 人类 此外，肺炎衣原体与慢性 呼吸系统疾病和一些免疫病理学疾病。 的 肺炎衣原体感染触发这些 免疫病理学疾病在很大程度上是未知的。 临床谱 肺炎衣原体感染已扩展到动脉粥样硬化， 相关的临床表现，如冠心病， 美国头号杀手。通过多种方法，证据表明， 在整个动脉树的粥样硬化中发现了微生物。 如果 生物体被证明在动脉粥样硬化形成中起作用， 迫切需要确定候选疫苗并迅速开发 诊断方法 这些措施应有助于阐明 这些因素有助于肺炎衣原体的发病机制。 有一个 缺乏关于肺炎衣原体毒力因子的可用信息 以及预防感染的潜在目标。该提案将 通过分子、功能和抗原分析来确定 以下假定的毒力因子的作用：1）鉴定和 表征肺炎衣原体特异性抗体识别的抗原 单克隆抗体因为这种抗体识别表面抗原， 具有感染性中和活性， 肺炎衣原体; 2）鉴定可激发免疫应答的肺炎衣原体抗原 肺炎衣原体特异性中和抗体：98 kDa和42 kDa 含有肺炎衣原体特异性反应性的抗原 在人类感染过程中，在循环免疫系统中也发现了 冠状动脉疾病患者的复合物; 3）表征 一种新的衣原体属70-kDa白蛋白样蛋白， 中和表位 抗原模拟的假设将是 通过筛选从过去的研究中收集的血清来解决， 与衣原体感染有关的免疫病理学疾病。 的 这些研究的最终目标是阐明肺炎衣原体 有助于发病机制和免疫病理学的抗原， 指导今后关于制定预防措施的研究， 干预和诊断。