Chlamydia pneumoniae Antigens of Biological Significance

具有生物学意义的肺炎衣原体抗原

• 批准号: 7026454
• 负责人: LEE ANN CAMPBELL
• 金额: $ 33.31万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7026454
• 关键词: Chlamydia trachomatis; antireceptor antibody; atherosclerosis; bacterial antigens; bacterial pneumonia; bacterial vaccines; carbohydrate receptor; chlamydial disease; communicable disease diagnosis; confocal scanning microscopy; enzyme linked immunosorbent assay; flow cytometry; host organism interaction; human tissue; immunoaffinity chromatography; immunopathology; immunoprecipitation; laboratory mouse; mannose 6 phosphate; monoclonal antibody; polymerase chain reaction; serology /serodiagnosis; tissue /cell culture; vaccine development; virulence; western blottings; yeast two hybrid system

DESCRIPTION (provided by the applicant): Chlamydia pneumoniae is a human respiratory pathogen that causes 5 percent to 10 percent of pneumonia, bronchitis, and sinusitis. Virtually everyone is infected in his or her lifetime and reinfection is common. Infection is difficult to treat even with sensitive antibiotics. Chronic infection is common and has been associated with asthma, reactive airway disease, Reiter's syndrome, erythema nodosum, and sarcoidosis. The potential public health impact of infection with this pathogen is underscored by the association of C. pneumoniae with atherosclerosis and related clinical manifestations such as coronary heart disease, carotid artery stenosis, aortic aneurysm, claudication, and stroke. If C. pneumoniae infection plays a role in atherogenesis, there will be an urgent need to facilitate diagnosis and develop strategies for intervention and prevention. The overall goal of this proposal is two fold. First, C. pneumoniae specific antigens that are recognized during human infection will be exploited to facilitate serodiagnosis and identify putative vaccine candidates. The second goal is to define chlamydial/host cell interactions that lead to entry and survival of C. pneumoniae in host cells relevant to atherosclerosis. The specific focus will be on the interaction of the chlamydial glycan moiety with carbohydrate binding receptors on the host cell. Importantly, infection of epithelial cells can be inhibited with N-linked high mannose type oligosaccharide, the major component of the glycan. The novel hypothesis to be tested is that C. pneumoniae enters through the mannose-6 phosphate receptor by binding to the site involved in transport of phosphomannosylated residues to the lysosome and this differs from C. trachomatis, which utilizes the mannose receptor. The ultimate goals of these studies are to identify C. pneumoniae specific antigens to facilitate laboratory diagnosis and virulence factors playing a role in pathogenesis to guide vaccine development or develop anti-adhesive strategies for prevention of infection.

描述（由申请方提供）：肺炎衣原体是一种人源性肺炎衣原体。 导致5%到10%肺炎的呼吸道病原体， 支气管炎和鼻窦炎事实上，每个人都在他或她的 终身感染和再感染是常见的。感染很难治疗，即使 敏感抗生素慢性感染很常见， 哮喘、反应性气道疾病、Reiter综合征、结节性红斑，以及 结节病感染这种病原体的潜在公共卫生影响 C.肺炎伴动脉粥样硬化， 相关临床表现如冠心病、颈动脉 狭窄、主动脉瘤、跛行和中风。如果C.肺炎菌感染 在动脉粥样硬化形成中起作用，迫切需要促进 诊断和制定干预和预防战略。整体 这项建议有两个目的。第一，C.肺炎特异性抗原， 在人类感染过程中被识别出来， 血清学诊断和鉴定推定的候选疫苗。第二个目标是 定义衣原体/宿主细胞相互作用，导致进入和生存的C。 在与动脉粥样硬化相关的宿主细胞中，具体重点将 是衣原体聚糖部分与碳水化合物结合的相互作用 宿主细胞上的受体。重要的是，上皮细胞的感染可以是 抑制与N-连接的高甘露糖型寡糖，主要成分 的聚糖。有待检验的新假设是C。肺炎进入 通过甘露糖-6磷酸受体结合到参与 磷酸甘露糖基化残基转运到溶酶体，这不同于 C.沙眼衣原体，其利用甘露糖受体。的最终目标 这些研究是为了确定C.肺炎特异性抗原， 实验室诊断和毒力因子在发病机制中发挥作用， 指导疫苗开发或制定抗粘附战略， 感染