OVARIAN HORMONE REGULATION OF NA NEURONS

NA 神经元的卵巢激素调节

• 批准号: 2862221
• 负责人: MEREDITH A CURRAN
• 金额: $ 1.96万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-14 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2862221
• 关键词: animal tissue; behavioral /social science research tag; estradiol; estrogen receptors; female; histochemistry /cytochemistry; hormone regulation /control mechanism; in situ hybridization; locus coeruleus; luteinizing hormone; neuroendocrine system; neurons; norepinephrine; progesterone receptors; protein localization; sex behavior; solitary tract nucleus; tyrosine 3 monooxygenase

DESCRIPTION (applicant's abstract): Little is known about how specific noradrenergic (NA) cell groups involved in regulating ovulation and reproductive behaviors respond to estradiol and/or progesterone. Recently, it has been suggested that A1, A2, and possibly A6 brainstem NA nuclei are the major sources of NA to the preoptic area and hypothalamus. The present research is designed to examine 1) changes in tyrosine hydroxylase (TH: rate-limiting enzyme for NA synthesis) mRNA levels in relation to the onset of the preovulatory luteinizing hormone surge and the onset of female sexual behavior. In all experiments, I will use dual-label in situ hybridization histochemistry to colocalize TH mRNA and estrogen receptor (ER)-alpha, ER-beta, or progestin receptor (PR) mRNA. Additionally, I will determine changes in levels of TH mRNA at various times of the day of and the day after the LH surge and onset of lordosis behavior. The results of this project will provide new information regarding the interactions between ovarian hormones and the NA system, providing a better understanding of the mechanisms by which steroid hormones influence reproductive functions.

描述（申请人摘要）：关于参与调节排卵和生殖行为的特定去甲肾上腺素能（NA）细胞群对雌二醇和/或孕酮的反应知之甚少。最近，有人提出，A1，A2，可能A6脑干NA核团是视前区和下丘脑NA的主要来源。本研究旨在研究1）酪氨酸羟化酶（TH：NA合成的限速酶）mRNA水平的变化与排卵前促黄体生成素峰的开始和女性性行为的开始的关系。在所有实验中，我将使用双标记原位杂交组织化学共定位TH mRNA和雌激素受体（ER）-α，ER-β，或孕激素受体（PR）mRNA。此外，我将测定LH峰和脊柱前凸行为发生当天和次日不同时间TH mRNA水平的变化。该项目的结果将提供有关卵巢激素和NA系统之间相互作用的新信息，从而更好地了解类固醇激素影响生殖功能的机制。