OVARIAN HORMONE REGULATION OF NA NEURONS

NA 神经元的卵巢激素调节

• 批准号: 6185672
• 负责人: MEREDITH A CURRAN
• 金额: $ 1.79万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-14 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6185672
• 关键词: animal tissue; behavioral /social science research tag; estradiol; estrogen receptors; female; histochemistry /cytochemistry; hormone regulation /control mechanism; in situ hybridization; locus coeruleus; luteinizing hormone; neuroendocrine system; neurons; norepinephrine; progesterone receptors; protein localization; sex behavior; solitary tract nucleus; tyrosine 3 monooxygenase

DESCRIPTION (applicant's abstract): Little is known about how specific noradrenergic (NA) cell groups involved in regulating ovulation and reproductive behaviors respond to estradiol and/or progesterone. Recently, it has been suggested that A1, A2, and possibly A6 brainstem NA nuclei are the major sources of NA to the preoptic area and hypothalamus. The present research is designed to examine 1) changes in tyrosine hydroxylase (TH: rate-limiting enzyme for NA synthesis) mRNA levels in relation to the onset of the preovulatory luteinizing hormone surge and the onset of female sexual behavior. In all experiments, I will use dual-label in situ hybridization histochemistry to colocalize TH mRNA and estrogen receptor (ER)-alpha, ER-beta, or progestin receptor (PR) mRNA. Additionally, I will determine changes in levels of TH mRNA at various times of the day of and the day after the LH surge and onset of lordosis behavior. The results of this project will provide new information regarding the interactions between ovarian hormones and the NA system, providing a better understanding of the mechanisms by which steroid hormones influence reproductive functions.

描述（申请人摘要）：关于参与调节排卵和生殖行为的特定去甲肾上腺素能（NA）细胞群如何对雌二醇和/或黄体酮做出反应，我们知之甚少。最近有研究表明，A1、A2和可能的A6脑干NA核是视前区和下丘脑NA的主要来源。本研究旨在探讨1)酪氨酸羟化酶（TH: NA合成的限速酶）mRNA水平的变化与排卵前黄体生成素激增和女性性行为发生的关系。在所有实验中，我将使用双标记原位杂交组织化学共定位TH mRNA和雌激素受体(ER)- α， ER- β或黄体酮受体（PR） mRNA。此外，我将确定在LH激增和前凸行为发生的当天和之后一天的不同时间TH mRNA水平的变化。本项目的研究结果将为卵巢激素与NA系统的相互作用提供新的信息，更好地了解类固醇激素影响生殖功能的机制。