STRUCTURE AND FUNCTION OF NUCLEAR RNP PARTICLES

信使RNA(MRNAs)是在真核细胞的细胞核中形成的，通过 初级RNA聚合酶II的广泛转录后加工 成绩单。这些转录本被称为异质核RNA(HnRNAs) 或前mRNAs，在整个过程中都与特定的蛋白质相关 它们位于原子核中。结合蛋白质的统称 HnRNAs及其核加工产品，且不稳定 其他类别的RNP复合体的成分，如SnRNPs，是hnRNP 蛋白质。HnRNP蛋白是Avid RNA结合蛋白， 核内hnRNA的加工是在hnRNA蛋白(HnRNP)中进行的 复合体。HnRNP蛋白的意义在于它们影响 HnRNA的结构和相互作用及其加工成mRNAs。AS 几种hnRNP蛋白在细胞核和细胞质之间穿梭，它们 也可能在细胞质和核中具有新的功能- MRNAs的细胞质运输。主要的hnRNP蛋白是 因此，细胞核和hnRNP复合体中最丰富的蛋白质也是 有兴趣是因为它们是主要的核结构。为了 了解基因表达的转录后途径 在细胞中运行，我们研究了结构、功能和 HnRNP蛋白的定位。尽管在以下方面取得了很大进展 在过去的几年里，这些领域有很大的基本面 其重要性仍有待了解。中提出的研究重点 这项资助申请是关于进一步的分子表征 HnRNP蛋白质，包括其序列、结构、 RNA结合活性，蛋白质-蛋白质相互作用，组装成 复合体、生化活性和在信使核糖核酸生物发生中的作用。

Messenger RNAs (mRNAs) are formed in the nuclei of eukaryotic cells by extensive posttranscriptional processing of primary RNA polymerase II transcripts. These transcripts, called heterogeneous nuclear RNAs (hnRNAs) or pre-mRNAs, are associated with specific proteins throughout the time they are in the nucleus. The collective term for the proteins that bind hnRNAs and their nuclear processing products, and which are not stable components of other classes of RNP complexes such as snRNPs, is hnRNP proteins. The hnRNP proteins are avid RNA-binding proteins and the processing of hnRNA in the nucleus takes place in hnRNA-protein (hnRNP) complexes. The significance of hnRNP proteins is that they influence the structure and interactions of hnRNAs and their processing into mRNAs. As several hnRNP proteins shuttle between the nucleus and the cytoplasm, they may also have novel functions in the cytoplasm as well as in nucleo- cytoplasmic transport of mRNAs. The major hnRNP proteins are among the most abundant proteins in the nucleus and hnRNP complexes are thus also of interest because they are major nuclear structures. In order to understand how the post-transcriptional pathway of gene expression operates in the cell, we investigate the structure, function, and localization of the hnRNP proteins. Though much progress has been made in these areas over the past several years, a great deal of fundamental importance remains to be learned. The focus of the research proposed in this grant application is on the further molecular characterization of the hnRNP proteins, including determination of their sequences, structures, RNA-binding activities, protein-protein interactions, assembly into complexes, biochemical activities, and functions in mRNA biogenesis.