DIFFERENTIAL EXPRESSION OF DIVERSE PLANT ACTIN GENES
多种植物肌动蛋白基因的差异表达
基本信息
- 批准号:6039284
- 负责人:
- 金额:$ 8.17万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1986
- 资助国家:美国
- 起止时间:1986-04-01 至 1999-12-31
- 项目状态:已结题
- 来源:
- 关键词:Arabidopsis actin binding protein actins antisense nucleic acid fusion gene gene induction /repression gene mutation genetic mapping genetic promoter element introns messenger RNA plant genetics polymerase chain reaction protein protein interaction protein structure function reporter genes site directed mutagenesis structural genes tissue mosaicism transposon /insertion element yeasts
项目摘要
Our research is focused on the hypothesis that the ancient and divergent
classes of plant actin genes have been preserved throughout vascular plant
evolution because they have unique patterns of gene regulation and/or
encode proteins with unique functions. During the last grant period we
isolated, sequenced, and characterized the ten members of the Arabidopsis
actin gene family (ACT1, 2, 3, 4, 5, 7, 8, 9psi, 11, & 12). They fall into
six subclasses which were approximately as divergent as vertebrate muscle
actin is from cytoplasmic actin, but the plant proteins had much higher
levels of charged residue variability. Based on gene specific Northerns
and RT-PCR analysis of RNA expression and 5' region/reporter fusions
examined in hundreds of independent transgenic plants, eight of the genes
in five subclasses were expressed at very high levels in at least one cell
type. These five subclasses had very distinct and different expression
patterns covering all the tissues and developmental stages of Arabidopsis
(e.g. the ACT2/ACT8 subclass was constitutive in vegetative tissues, while
the ACT1/ACT3 subclass was expressed in organ primordia and mature
pollen). An initial characterization of Arabidopsis profilin sequences
demonstrated the presence of a large and complex gene family which
included constitutive (PRF1, 2, 3) and pollen specific members (PRF4). In
light of these and other data on the diverse plant actins and profilins we
propose a second hypothesis for our future work: there are concordantly
expressed subclasses of actins and actin binding proteins (e.g.
profilins), which have evolved distinct protein-protein interactions.
Based on these two hypotheses our specific aims for the next grant period
are: (l) to demonstrate that the various actin genes or subfamilies are
required for normal Arabidopsis growth and development by characterizing
the phenotype of plants deficient in actin expression (from a T-DNA
insertion library and by antisense suppression and co-suppression); (2) To
determine if the major cis-elements controlling tissue specific expression
are in the promoter region, mRNA leader or leader intron; (3) to determine
the detailed expression patterns of a few representative members of the
diverse profilin subclasses (i.e. PRF1 and PRF4); (4) to initiate
experiments which will connect the origin of the diverse actin lineages
with macroevolutionary events (e.g. ACT1 with the origin of meristems);
and (5) to compare the strength of protein-protein interaction between
actin-profilin pairs that are co-expressed, with those that are not. This
work will expand our basic understanding of the eukaryotic cytoskeleton
and enhance our ability to manipulate the properties of higher plants.
我们的研究主要集中在古代和分化的假设上
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Richard Brian Meagher其他文献
Richard Brian Meagher的其他文献
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{{ truncateString('Richard Brian Meagher', 18)}}的其他基金
Targeted pan-antibacterial liposomes to control pathogenic mycobacteria
靶向泛抗菌脂质体控制致病性分枝杆菌
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10569282 - 财政年份:2023
- 资助金额:
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10668509 - 财政年份:2022
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Targeted delivery of antifungal drug loaded liposomes to control mucormycosis
靶向递送抗真菌药物脂质体来控制毛霉菌病
- 批准号:
10509943 - 财政年份:2022
- 资助金额:
$ 8.17万 - 项目类别:
Antifungal Liposomes Targeted to Cell Wall Mannans
针对细胞壁甘露聚糖的抗真菌脂质体
- 批准号:
9979038 - 财政年份:2020
- 资助金额:
$ 8.17万 - 项目类别:
NUCLEAR CAPTURE FOR CELL-TYPE SPECIFIC EPIGENETIC ANALYSIS OF ADIPOCYTES
用于脂肪细胞细胞类型特异性表观遗传分析的核捕获
- 批准号:
8760476 - 财政年份:2014
- 资助金额:
$ 8.17万 - 项目类别:
NUCLEAR CAPTURE FOR CELL-TYPE SPECIFIC EPIGENETIC ANALYSIS OF ADIPOCYTES
用于脂肪细胞细胞类型特异性表观遗传分析的核捕获
- 批准号:
8917210 - 财政年份:2014
- 资助金额:
$ 8.17万 - 项目类别:
Differential Expression of the Diverse Plant Actins
多种植物肌动蛋白的差异表达
- 批准号:
7931495 - 财政年份:2009
- 资助金额:
$ 8.17万 - 项目类别:
DIFFERENTIAL EXPRESSION OF DIVERSE PLANT ACTIN GENES
多种植物肌动蛋白基因的差异表达
- 批准号:
2684820 - 财政年份:1986
- 资助金额:
$ 8.17万 - 项目类别:
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