MRNA DEGRADATION AND TRANSLATION CONTROL IN YEAST

描述(改编自申请者摘要)：总体目标 这项建议是为了了解转录降解途径和 它与翻译控制的关系，使用发芽酵母S。 作为一个模型系统的啤酒。重点放在XRN1基因上，编码 酵母的主要5‘-3’外切核糖核酸酶活性，被认为是 RNA周转途径后期的主要分解代谢酶。 研究人员打算通过以下方式确定该途径中的其他基因 筛选具有xrn等位基因的合成致命性突变 (目标1)并鉴定获得的突变体(包括已经 已知的Ski2基因)在体内的RNA周转和翻译(AIM 2)和 体外培养(目标4)。目标3针对XRN1的靶向突变以 分析其功能域并获得显性负突变。 出现了许多与RNA周转和翻译控制有关的蛋白质 在真核生物中高度保守，被认为有助于细胞 应对病原体感染和肿瘤形成等挑战。

DESCRIPTION (Adapted from applicant's abstract): The overall goal of this proposal is to understand the transcript degradation pathway and its relationship to translational control, using the budding yeast S. cerevisiae as a model system. The focus is on the XRN1 gene, encoding the major 5'-3' exoribonuclease activity of yeast and believed to be the major catabolic enzyme in the late stages of the RNA turnover pathway. The investigator intends to identify other genes in the pathway by screening for mutations that are synthetically lethal with xrn alleles (Aim 1) and to characterize the mutants obtained (including the already known SKI2 gene) in RNA turnover and translation, in vivo (Aim 2) and in vitro (Aim 4). Aim 3 is directed at targeted mutagenesis of XRN1 to analyze its functional domains and obtain dominant negative mutations. Many proteins involved in RNA turnover and translation control appear highly conserved among eukaryotes and are thought to help the cell respond to such challenges as pathogenic infection and oncogenesis.