RNA LOCALIZATION IN XENOPUS OOCYTES

描述(改编自摘要)：极性的确立 在卵子中可以被视为胚胎中最早的一步 图案化。因此，早期卵裂之间细胞命运的差异 细胞是不对称分布的结果 受精前卵子细胞质中的信息分子。 这种极性的基础是局域化的母体 以蛋白质或信使核糖核酸形式的决定因素。趁有机会 在许多情况下存在局部行列式的证据 关于系统的本地化进程，人们仍然知之甚少。这 研究提案通过调查以下内容来解决此问题 利用青蛙研究基因定位的生物化学机制 非洲爪哇作为典范。PI纯化并克隆了一个 基本定位因子，已确定的其他蛋白质是 潜在的本地化因素，并定义了这些关键因素 RNA定位信号中的序列。以下是具体的 研究目的：(1)VgRBP60的特性 定位因子；(2)信使核糖核酸机制的研究 定位；(3)其他RNA-蛋白质相互作用的分析 介导蛋白的定位、纯化和鉴定 本地化机制的其他蛋白质成分。

DESCRIPTION (Adapted from the abstract): Establishment of polarity in the egg can be viewed as the earliest step in embryonic patterning. Thus, differences in cell fate among the early cleavage cells are a consequence of asymmetric distributions of informational molecules in the egg cytoplasm before fertilization. The basis for such polarity is provided by localized maternal determinants in the form of protein or mRNA. While there is evidence for the existence of localized determinants in many systems, the localization process is still poorly understood. This research proposal addresses this problem by investigating the biochemical mechanisms underlying mRNA localization, using the frog Xenopus laevis as a model. The PI has purified and cloned an essential localization factor, identified other proteins that are potential localization factors, and defined these critical sequences within an RNA localization signal. The following specific aims are proposed: (1) characterization of VgRBP60, the essential localization factor; (2) investigation of the mechanism of mRNA localization; (3) analysis of other RNA-protein interactions mediating localization, and purification and characterization of other protein components of the localization machinery.