RNA Localization in Xenopus Oocytes
非洲爪蟾卵母细胞中的 RNA 定位
基本信息
- 批准号:8197643
- 负责人:
- 金额:$ 35.66万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1993
- 资助国家:美国
- 起止时间:1993-09-01 至 2014-11-30
- 项目状态:已结题
- 来源:
- 关键词:BindingCell PolarityCellsComplexCongenital AbnormalityCytoplasmCytoplasmic GranulesDefectDevelopmentEmbryoEmbryonic DevelopmentFoundationsFragile X SyndromeGoalsGrowth FactorHealthHumanIn VitroInvestigationLicensingLinkMessenger RNAModelingMolecularMolecular MotorsMotorNervous System PhysiologyNuclearOogenesisPathway interactionsPatternPeptidesPlayProcessProtein BiosynthesisProteinsRNARNA TransportRecruitment ActivityResearchResearch Project GrantsRibonucleoproteinsRoleSignal TransductionSomatic CellSpinal Muscular AtrophyTranslational RepressionTransport ProcessVertebratesWorkXenopus oocyteblastomere structuredesignegghuman diseasein vivoinsightmRNA Expressionnervous system disorderprotein expressionprotein protein interactionresearch study
项目摘要
Project Summary
Cytoplasmic RNA localization is a widespread mechanism for generating both embryonic and somatic cell
polarity. Among vertebrates, Vg1 mRNA is a prominent example of a localized mRNA that plays a role in
embryonic patterning. Vg1 mRNA encodes a peptide growth factor, and is localized during oogenesis to the
vegetal cytoplasm of Xenopus oocytes. Restricted expression of Vg1 protein in the vegetal hemisphere of the
egg is critical for correct patterning of the embryo, making localization of Vg1 mRNA an important model for
understanding how maternal molecules are localized to influence pattern and polarity.
The goal of this research project is to investigate how RNA molecules can be targeted to specific regions of
the cell cytoplasm to generate cell polarity through spatially restricted protein expression. The foundation for
this investigation has been laid by our progress studying the molecular interactions that govern this process.
Our experiments have defined critical RNA-protein and protein-protein interactions leading to assembly of a
transport-competent RNP complex and have uncovered distinct steps in the localization pathway. Our current
proposal seeks to extend these findings by studying the dynamic transitions between these steps in the
localization pathway. We will use a combination of in vivo and in vitro approaches to determine the molecular
mechanisms responsible for localized expression of Vg1 mRNA. Three specific aims are proposed: In Aim I,
we will characterize the dynamics and transport of the cytoplasmic Vg1 RNP complex, in Aim II, we will
analyze the assembly and activity of RNP transport granules and in Aim III we will investigate the mechanisms
that couple translational repression and RNA localization
The proposed research is designed to reveal the mechanisms by which mRNA molecules are transported
within cells to generate spatially restricted protein expression, and will provide insight into how developmental
signals are spatially distributed in the vertebrate embryo. This work will impact issues related to human health
such as birth defects, as well as neurological diseases that have been linked to defective RNA transport and
localized protein synthesis.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Kimberly L. Mowry其他文献
RNA transport in the oocyte cytoplasm: How to get there from here
- DOI:
10.1016/j.ydbio.2008.05.011 - 发表时间:
2008-07-15 - 期刊:
- 影响因子:
- 作者:
Kimberly L. Mowry;James A. Gagnon;Jill A. Kreiling;Timothy J. Messitt;Catherine A. Pratt;Young J. Yoon - 通讯作者:
Young J. Yoon
Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in emXenopus/em oocytes
与 RNA 的多价相互作用驱动了 emXenopus/em 卵母细胞中生物分子凝聚体的募集和动态变化。
- DOI:
10.1016/j.isci.2022.104811 - 发表时间:
2022-08-19 - 期刊:
- 影响因子:4.100
- 作者:
Sarah E. Cabral;Jessica P. Otis;Kimberly L. Mowry - 通讯作者:
Kimberly L. Mowry
Kimberly L. Mowry的其他文献
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{{ truncateString('Kimberly L. Mowry', 18)}}的其他基金
Training in Molecular and Cell Biology and Biochemistry
分子和细胞生物学和生物化学培训
- 批准号:
7889558 - 财政年份:2009
- 资助金额:
$ 35.66万 - 项目类别:
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