RNA Localization in Xenopus Oocytes

非洲爪蟾卵母细胞中的 RNA 定位

基本信息

  • 批准号:
    8197643
  • 负责人:
  • 金额:
    $ 35.66万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1993
  • 资助国家:
    美国
  • 起止时间:
    1993-09-01 至 2014-11-30
  • 项目状态:
    已结题

项目摘要

Project Summary Cytoplasmic RNA localization is a widespread mechanism for generating both embryonic and somatic cell polarity. Among vertebrates, Vg1 mRNA is a prominent example of a localized mRNA that plays a role in embryonic patterning. Vg1 mRNA encodes a peptide growth factor, and is localized during oogenesis to the vegetal cytoplasm of Xenopus oocytes. Restricted expression of Vg1 protein in the vegetal hemisphere of the egg is critical for correct patterning of the embryo, making localization of Vg1 mRNA an important model for understanding how maternal molecules are localized to influence pattern and polarity. The goal of this research project is to investigate how RNA molecules can be targeted to specific regions of the cell cytoplasm to generate cell polarity through spatially restricted protein expression. The foundation for this investigation has been laid by our progress studying the molecular interactions that govern this process. Our experiments have defined critical RNA-protein and protein-protein interactions leading to assembly of a transport-competent RNP complex and have uncovered distinct steps in the localization pathway. Our current proposal seeks to extend these findings by studying the dynamic transitions between these steps in the localization pathway. We will use a combination of in vivo and in vitro approaches to determine the molecular mechanisms responsible for localized expression of Vg1 mRNA. Three specific aims are proposed: In Aim I, we will characterize the dynamics and transport of the cytoplasmic Vg1 RNP complex, in Aim II, we will analyze the assembly and activity of RNP transport granules and in Aim III we will investigate the mechanisms that couple translational repression and RNA localization The proposed research is designed to reveal the mechanisms by which mRNA molecules are transported within cells to generate spatially restricted protein expression, and will provide insight into how developmental signals are spatially distributed in the vertebrate embryo. This work will impact issues related to human health such as birth defects, as well as neurological diseases that have been linked to defective RNA transport and localized protein synthesis.
项目总结

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Kimberly L. Mowry其他文献

RNA transport in the oocyte cytoplasm: How to get there from here
  • DOI:
    10.1016/j.ydbio.2008.05.011
  • 发表时间:
    2008-07-15
  • 期刊:
  • 影响因子:
  • 作者:
    Kimberly L. Mowry;James A. Gagnon;Jill A. Kreiling;Timothy J. Messitt;Catherine A. Pratt;Young J. Yoon
  • 通讯作者:
    Young J. Yoon
Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in emXenopus/em oocytes
与 RNA 的多价相互作用驱动了 emXenopus/em 卵母细胞中生物分子凝聚体的募集和动态变化。
  • DOI:
    10.1016/j.isci.2022.104811
  • 发表时间:
    2022-08-19
  • 期刊:
  • 影响因子:
    4.100
  • 作者:
    Sarah E. Cabral;Jessica P. Otis;Kimberly L. Mowry
  • 通讯作者:
    Kimberly L. Mowry

Kimberly L. Mowry的其他文献

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{{ truncateString('Kimberly L. Mowry', 18)}}的其他基金

Training in Molecular and Cell Biology and Biochemistry
分子和细胞生物学和生物化学培训
  • 批准号:
    7889558
  • 财政年份:
    2009
  • 资助金额:
    $ 35.66万
  • 项目类别:
RNA Localization in Xenopus Ooctytes
非洲爪蟾卵细胞中的 RNA 定位
  • 批准号:
    6870635
  • 财政年份:
    1993
  • 资助金额:
    $ 35.66万
  • 项目类别:
RNA Localization in Xenopus Ooctytes
非洲爪蟾卵细胞中的 RNA 定位
  • 批准号:
    6987894
  • 财政年份:
    1993
  • 资助金额:
    $ 35.66万
  • 项目类别:
RNA Localization in Xenopus Oocytes
非洲爪蟾卵母细胞中的 RNA 定位
  • 批准号:
    7997247
  • 财政年份:
    1993
  • 资助金额:
    $ 35.66万
  • 项目类别:
Mechanisms of RNA localization in oocytes
RNA在卵母细胞中的定位机制
  • 批准号:
    8974969
  • 财政年份:
    1993
  • 资助金额:
    $ 35.66万
  • 项目类别:
RNA Localization in Xenopus Ooctytes
非洲爪蟾卵细胞中的 RNA 定位
  • 批准号:
    7149168
  • 财政年份:
    1993
  • 资助金额:
    $ 35.66万
  • 项目类别:
RNA LOCALIZATION IN XENOPUS OOCYTES
非洲爪蟾卵母细胞中的 RNA 定位
  • 批准号:
    2855615
  • 财政年份:
    1993
  • 资助金额:
    $ 35.66万
  • 项目类别:
RNA LOCALIZATION IN XENOPUS OOCYTES
非洲爪蟾卵母细胞中的 RNA 定位
  • 批准号:
    2853657
  • 财政年份:
    1993
  • 资助金额:
    $ 35.66万
  • 项目类别:
Mechanisms of RNA localization in oocytes
RNA在卵母细胞中的定位机制
  • 批准号:
    9107469
  • 财政年份:
    1993
  • 资助金额:
    $ 35.66万
  • 项目类别:
RNA LOCALIZATION IN XENOPUS OOCYTES
非洲爪蟾卵母细胞中的 RNA 定位
  • 批准号:
    2203032
  • 财政年份:
    1993
  • 资助金额:
    $ 35.66万
  • 项目类别:

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