MECHANISMS OF GENERATION OF GROWTH CONE CALCIUM WAVES

生长锥钙波的产生机制

• 批准号: 2697950
• 负责人: NICHOLAS CANADAY SPITZER
• 金额: $ 16.02万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-08 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2697950
• 关键词: Xenopus; calcium flux; calcium indicator; developmental neurobiology; growth cones; laminin; membrane transport proteins; neurogenesis; ryanodine; voltage /patch clamp

DESCRIPTION (modified from applicant's abstract) This project focuses on spontaneous elevations of intracellular calcium in growth cones (calcium waves) that regulate extension of neurites. The outgrowth of neurites to establish specific neuronal connections is an important aspect of early development of the nervous system. The research addresses the mechanisms of generation of calcium waves in growth cones of amphibian spinal neurons in culture and has two major specific aims. The first aim is to investigate the contributions of a potentially novel calcium leak pathway in combination with known calcium transporters to the generation of calcium waves. The second aim is to determine the identify and roles of intracellular calcium stores in amplifying elevations of intracellular calcium in growth cones and thereby regulating neurite extension. Optical imaging of intracellular calcium and recording of ionic currents will be used to address these aims.

描述（根据申请人摘要修改） 该项目的重点是自发升高的细胞内钙， 生长锥（钙波），调节神经突的延伸。的 神经突的生长以建立特定的神经元连接是一种 神经系统早期发育的重要方面。研究 提出了生长锥中钙波产生的机制， 两栖类脊髓神经元的培养，并有两个主要的具体目标。的 第一个目的是调查一个潜在的新的贡献， 钙渗漏途径与已知的钙转运蛋白结合 钙波的产生。第二个目的是确定 以及细胞内钙储存在放大 生长锥中的细胞内钙，从而调节神经突 扩展名.细胞内钙离子的光学成像和离子记录 电流将用于实现这些目标。