MECHANISMS OF GENERATION OF GROWTH CONE CALCIUM WAVES

描述(修改自申请人的摘要) 本项目的重点是细胞内钙的自发升高。 调节神经突起延伸的生长锥体(钙波)。这个 神经突起的生长建立特定的神经元连接是一种 神经系统早期发育的重要方面。这项研究 阐述了生长锥体内钙波的产生机制。 两栖类脊髓神经元的培养有两大具体目的。这个 第一个目标是调查一部潜在的小说的贡献 钙泄漏途径与已知的钙转运体相结合 产生钙波。第二个目标是确定身份 以及细胞内钙储存在提高血管紧张素转换酶活性中的作用 生长锥体内的细胞内钙离子，从而调节轴突 分机。细胞内钙离子的光学成像和离子记录 将利用电流来实现这些目标。

DESCRIPTION (modified from applicant's abstract) This project focuses on spontaneous elevations of intracellular calcium in growth cones (calcium waves) that regulate extension of neurites. The outgrowth of neurites to establish specific neuronal connections is an important aspect of early development of the nervous system. The research addresses the mechanisms of generation of calcium waves in growth cones of amphibian spinal neurons in culture and has two major specific aims. The first aim is to investigate the contributions of a potentially novel calcium leak pathway in combination with known calcium transporters to the generation of calcium waves. The second aim is to determine the identify and roles of intracellular calcium stores in amplifying elevations of intracellular calcium in growth cones and thereby regulating neurite extension. Optical imaging of intracellular calcium and recording of ionic currents will be used to address these aims.