CORE--TRANSGENIC MOUSE

描述：(申请者描述)共享的转基因小鼠 资源提供与生产和维护相关的五项服务 转基因和基因敲除小鼠品系。这些措施包括： 1)为SACI成员培育转基因小鼠新品系。 研究人员向核心提供了一种含有所需 转基因和详细的质粒图。核心负责 转基因片段的分离、制备及鉴定 转基因创始小鼠。至少有两只创始小鼠是为 每一次转基因。2)通过胚胎移植消除小鼠品系的再衍生 从外部非商业性获取的小鼠的病原体 消息来源。该方法效率高，大大降低了成本和成本。 隔离方法消除病原体所需的时间。 3)小鼠石灰胚胎超低温保存。这种方法提供了一种方法来 不活跃繁殖和保护的小鼠品系的保存 防止因疾病而丧失小鼠品系。 4)胚泡注射胚胎干细胞，以生产 击倒鼠标线。这表示要添加到 转基因小鼠共享资源调查人员为ES提供核心 一种特定基因被同源基因干扰的细胞系 重组。CORE将这些细胞显微注射到小鼠囊胚中以 制造嵌合体小鼠。核心还将与正在开发的专家系统进行协调 和饲养细胞系，靶向载体，关于ES细胞的方案和建议 培养和操纵。 5)咨询服务。核心员工提供关于一般情况的建议 转基因和基因敲除方法与质粒构建。为 缺乏转基因小鼠经验的实验室，说明是 在小鼠繁殖、断奶、打耳和尾巴DNA上提供 与世隔绝。该核心还在准备应用程序方面提供帮助 UTHSCSA IACUC在研究中使用实验动物。

Description: (Applicant's Description) The Transgenic Mouse Shared Resource provides five services related to the production and maintenance of transgenic and knock-out mouse lines. These include: 1) The production of new lines of transgenic mice for SACI members. Investigators provide the core with a plasmid containing the desired transgene and a detailed plasmid map. The core is responsible for isolation of the transgene fragment and the production and identification of transgenic founder mice. At least two founder mice are produced for each transgene. 2) Mouse line rederivation by embryo transfer to eliminate pathogens from mice which have been obtained from outside, non-commercial sources. This method is highly effective and greatly reduces the cost and time involved in the quarantine approach to pathogen elimination. 3) Mouse lime embryo cryopreservation. This approach provides the means to preserve lines of mice without active breeding as well as safeguarding against the loss of mouse lines due to disease. 4) Blastocyst injection of embryonic stem (ES) cells for the production of knock-out mouse lines. This represents a new service to be added to the Transgenic Mouse Shared Resource Investigators provide the core with ES cell lines in which a specific gene has been disrupted by a homologous recombination. The core microinjects these cells into mouse blastocysts to produce chimeric mice. The core will also coordinate with a developing ES and feeder cell lines, targeting vectors, protocols and advice on ES cell culturing and manipulation. 5) Consulting services. The core staff provides advice on general transgenic and knock-out approaches and plasmid construction. For laboratories lacking experience with transgenic mice, instruction is provided on breeding of mice, weaning, ear punching, and tail DNA isolation. The core also provides assistance in preparing applications to the UTHSCSA IACUC for the use of laboratory animals in research.