Transgenic/Knockout Mouse Shared Resource

转基因/敲除小鼠共享资源：项目 总结/摘要 基因工程小鼠模型（GEMM）已成为癌症研究中不可或缺的工具， 它们的效用范围从肿瘤发生和发展的基础生物学研究到 基因精确的肿瘤模型，用于评估新的治疗方法。的总体目标 因此，转基因/敲除小鼠共享资源（TG-KO）有助于及时和经济有效地 弗吉尼亚联邦大学成员在癌症研究中生产和使用GEMM (VCU)梅西癌症中心（MCC）。这一目标将通过生产新型GEMM（包括 转基因、敲除、条件性敲除和敲入小鼠），使用常规和CRISPR/Cas9- 的技术，并提供全面的支持服务，以促进这些模型的使用， 包括繁殖群体管理、小鼠基因分型、小鼠品系再衍生、胚胎和精子 冷冻保存和冷冻复苏，以及咨询。 TG-KO由Jolene Windle博士执导，她拥有近30年的制作经验， 在癌症研究中使用GEMM，并得到2.5名额外的全职员工的支持，他们在以下方面提供了额外的专业知识： GEMM的创建，涉及GEMM生产和筛选的分子方法，以及育种菌落 管理TG-KO是一个联合管理的（MCC和VCU）资源，步行距离很近 MCC成员实验室。TG-KO设施包括限制进入的实验室和动物饲养 分子医学研究大楼屏障动物园的空间，以及一般的分子 生物实验室空间在下面的地板上，所有这些都配备了所提供的服务。TG-KO 没有固定的工作时间;但是，一部分工作人员通常从上午8：30开始工作 直到下午6：00或更晚，周一至周五。所有员工都可以通过电子邮件随时联系到， 他们通常会尽快回复调查人员的电子邮件，通常是在正常工作时间之外。 TG-KO提供的服务被跟踪并收费。其他支持由 机构来源和癌症中心支持赠款，这使得TG-KO提供其服务， 费率大大低于商业供应商的收费。TG提供的服务- KO通过提供实际上无法获得或成本高昂的必要服务，为MCC的研究增加价值 禁止通过替代供应商，包括其他非营利机构。通过结合在- TG-KO拥有专业知识和服务，价格极具竞争力，极大地促进了GEMM的使用， MCC成员在他们的基础和转化癌症研究工作。在CY 2015中，TG-KO为以下客户提供服务： 18个MCC成员，占用户群的64%，占所有充值的67%。

TRANSGENIC/KNOCKOUT MOUSE SHARED RESOURCE: PROJECT SUMMARY/ABSTRACT Genetically engineered mouse models (GEMMs) have become indispensable tools in cancer research, with their utility ranging from studies of the basic biology of tumorigenesis and progression to the creation of genetically accurate tumor models for the evaluation of novel therapeutic approaches. The overall objective of the Transgenic/Knockout Mouse Shared Resource (TG-KO) is therefore to facilitate timely and cost-effective production and use of GEMMs in cancer research by members of the Virginia Commonwealth University (VCU) Massey Cancer Center (MCC). This objective will be met by producing novel GEMMs (including transgenic, knockout, conditional knockout and knock-in mice) using both conventional and CRISPR/Cas9- based technologies and by providing a full range of support services to facilitate the use of these models, including breeding colony management, mouse genotyping, mouse line rederivation, embryo and sperm cryopreservation and cryorecovery, and consultation. The TG-KO is directed by Jolene Windle, PhD, who has nearly 30 years of experience in the production and use of GEMMs in cancer research, and is supported by 2.5 additional FTEs who bring added expertise in GEMM creation, molecular methods involved in GEMM production and screening, and breeding colony management. The TG-KO is a jointly managed (MCC and VCU) resource that is within close walking distance of MCC member laboratories. The TG-KO facility includes restricted-access laboratory and animal holding space in the barrier vivarium in the Molecular Medicine Research Building, as well as general molecular biology laboratory space on the floor below, all of which is fully equipped for the services provided. The TG-KO does not have fixed hours of operation; however, a subset of staff members is generally present from 8:30 AM until 6:00 PM or later, Monday through Friday. All members of the staff can be readily reached by email, and they typically respond to emails from investigators as soon as possible, often outside of regular work hours. The services provided by the TG-KO are tracked and charged back. Additional support is provided by institutional sources and the Cancer Center Support Grant, which allows the TG-KO to offer its services at rates that are significantly lower than those charged by commercial vendors. The services provided by the TG- KO add value for MCC research by providing necessary services that are not practically obtained or are cost prohibitive through alternative vendors, including other non-profit institutions. Through the combination of in- house expertise and services and highly competitive rates, the TG-KO greatly facilitates the use of GEMMs by MCC members in their basic and translational cancer research efforts. In CY2015 TG-KO provided services to 18 MCC members, corresponding to 64% of the user base and 67% of all recharges.