CORE--HYBRIDOMA LIBRARY / MONOCLONAL ANTIBODY PRODUCTION

核心--杂交瘤文库/单克隆抗体生产

• 批准号: 6101991
• 负责人: MICHAEL W FANGER
• 金额: --
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6101991
• 关键词: antibody formation; biomedical facility; cell line; hybridomas; monoclonal antibody; neoplasm /cancer; oncology; tissue /cell culture

The Hybridoma Library/Monoclonal Antibody Production Shared Resource (Hybridoma Shared Resource) provides NCCC investigators with quality-controlled monoclonal antibodies (mAbs) as purified proteins an d/or supernatants from hybridoma cell lines that have been obtained from the ATCC or from individual investigators. This facility maintains characterized and quality-controlled stocks of supematants and monoclonal antibodies from more than one hundred cell lines. It is especially cost- effective for studies that require only a small sample of a given antibody and would otherwise result in a charge to individual grants of $250 to $500 per antibody. This Resource also advises investigators on both the creation of new hybridomas and the production and purification of large quantities of antibodies as needed. During the past year this Resource was used exclusively by NCCC investigators; there is no charge-back system for supply of these antibodies, which often come from patented cell lines. Over the past year, this Shared Resource is estimated to have achieved a net savings of between $41,000 and $100,000 over the commercial cost of comparable antibodies.

杂交瘤文库/单克隆抗体生产共享资源（杂交瘤共享资源）为 NCCC 研究人员提供质量控制的单克隆抗体 (mAb)，作为从 ATCC 或个别研究人员获得的杂交瘤细胞系的纯化蛋白和 d/或上清液。该设施保存着来自一百多个细胞系的特征化和质量控制的上清液和单克隆抗体库存。对于只需要给定抗体的小样本的研究来说，它尤其具有成本效益，否则会导致每个抗体向个人拨款收取 250 至 500 美元的费用。该资源还为研究人员提供有关创建新杂交瘤以及根据需要生产和纯化大量抗体的建议。在过去的一年里，该资源仅由 NCCC 调查人员使用；这些抗体的供应没有退款系统，这些抗体通常来自专利细胞系。在过去的一年里，这一共享资源预计比同类抗体的商业成本净节省 41,000 美元至 100,000 美元。