STRUCTURAL ORGANIZATION OF THE MAMMALIAN GOLGI COMPLEX

高尔基复合体是糖脂生物合成的场所， 分泌物、血浆中糖蛋白的加工和分选步骤 膜和溶酶体/空泡蛋白。在哺乳动物细胞中，它 由紧凑的核旁复合体组成，其中个体 高尔基膜的浓度、高尔基堆叠通过以下方式相互连接 管状延伸物。这种核旁局部化是 细胞器相关的马达蛋白，可能是动力蛋白的成员 家族，以及微管相对于微管的方向 组织中心(MTOC)。在高尔基体内，细胞器是 以极化的方式组织，如通过形态、蛋白质 住所和功能。在本申请中，我们建议使用 用多标记方法探查脑池堆叠的关键方面 是团结一致的。这样的多标记物方法只有成为可能 通过建立稳定的表位转基因细胞系 标记高尔基糖基转移酶。尼尔森博士是这方面的先驱 这。

The Golgi complex is the site of glycolipid biosynthesis, finishing steps in glycoprotein processing and sorting of secretory, plasma membrane and lysosomal/vacuolar proteins. In mammalian cells, it consists of a compact, juxtanuclear complex in which individual concentrations of Golgi membranes, Golgi stacks, are interconnected by tubular extensions. This juxtanuclear localization is a consequence of organelle associated motor proteins, presumably members of the dynein family, and microtubule orientation with respect to the microtubule organizing center (MTOC). Within a Golgi stack, the organelle is organized in a polarized fashion as discerned by morphology, protein residence and function. In the present application, we propose to use a multimarker approach to probe key aspects of how the cisternal stack is held together. Such a multimarker approach has only become possible through the generation of stable cell lines transfected for epitope tagged Golgi glycosyltransferases. Dr. Nilsson has been a pioneer in this.