PHYTOMELATONIN AND CANCER PREVENTION

描述：这项研究将检验褪黑激素衍生的假设 来自松果体，受光循环调节或源自食物 （植物褪黑素），抑制雄性和雌性水牛大鼠的肝癌生长。 待测试的机制是褪黑激素减少肿瘤的摄取 促进生长的脂肪酸，亚油酸 (LA)，其代谢为 13-HODE （13-羟基-9,11-十八碳二烯酸），脂肪酸的表达 转运蛋白、FATP 和细胞谷胱甘肽 (GSH) 水平。 老鼠 将喂食补充有食品衍生或合成的纯化饮食 褪黑激素或将受到光循环。 血液褪黑激素水平 将被确定。 肿瘤生长和血液 A-V 脂肪酸差异和 将评估肿瘤脂肪酸摄取。 生化和分子 脂肪酸转运蛋白、FATP 和参与的酶的分析 13-HODE和GSH产量将被确定。 这个新颖的假设可能 提供有关褪黑激素对癌症影响的重要信息 通过抑制脂肪酸的摄取和代谢进行化学预防。

DESCRIPTION: This research will test the hypothesis that melatonin derived from the pineal gland, modulated by light cycling or derived from foods (phytomelatonin), inhibits hepatoma growth in male and female Buffalo rats. The mechanism to be tested is that melatonin reduces the uptake of the tumor growth-promoting fatty acid, linoleic acid (LA), its metabolism to 13-HODE (13-hydroxy-9,11-octadecadienoic acid), the expression of the fatty acid transport protein, FATP, and the level of cellular glutathione (GSH). Rats will be fed purified diets supplemented with food-derived or synthetic melatonin or will be subjected to light cycling. Blood melatonin levels will be determined. Tumor growth and blood A-V fatty acid differences and tumor fatty acid uptake will be assessed. Biochemical and molecular analysis of the fatty acid transporter, FATP, and of enzymes involved in 13-HODE and GSH production will be determined. This novel hypothesis may provide important information regarding the impact of melatonin on cancer chemoprevention via inhibition of fatty acid uptake and metabolism.