PHYTOMELATONIN AND CANCER PREVENTION

描述：这项研究将检验褪黑素源于 来自松果体的，受光循环调节的或来自食物的 (植物黑素)，抑制雄性和雌性水牛大鼠的肝癌生长。 有待测试的机制是褪黑素减少肿瘤的摄取 促生长脂肪酸亚油酸(LA)及其对13-HODE的代谢 (13-羟基-9，11-十八碳二烯酸)，脂肪酸的表达 转运蛋白、FATP和细胞谷胱甘肽(GSH)水平。老鼠 将喂给纯净的日粮，并添加食品衍生或合成 褪黑激素或会受到光循环的影响。血液褪黑素水平 将会被确定。肿瘤生长与血A-V脂肪酸的差异及 将对肿瘤脂肪酸摄取进行评估。生化与分子 分析脂肪酸转运体FATP和参与的酶 13-HODE和GSH的产量将被确定。这一新的假设可能 提供有关褪黑素对癌症影响的重要信息 通过抑制脂肪酸摄取和代谢来进行化学预防。

DESCRIPTION: This research will test the hypothesis that melatonin derived from the pineal gland, modulated by light cycling or derived from foods (phytomelatonin), inhibits hepatoma growth in male and female Buffalo rats. The mechanism to be tested is that melatonin reduces the uptake of the tumor growth-promoting fatty acid, linoleic acid (LA), its metabolism to 13-HODE (13-hydroxy-9,11-octadecadienoic acid), the expression of the fatty acid transport protein, FATP, and the level of cellular glutathione (GSH). Rats will be fed purified diets supplemented with food-derived or synthetic melatonin or will be subjected to light cycling. Blood melatonin levels will be determined. Tumor growth and blood A-V fatty acid differences and tumor fatty acid uptake will be assessed. Biochemical and molecular analysis of the fatty acid transporter, FATP, and of enzymes involved in 13-HODE and GSH production will be determined. This novel hypothesis may provide important information regarding the impact of melatonin on cancer chemoprevention via inhibition of fatty acid uptake and metabolism.